Document Detail


Assessment of aqueous humor dynamics in the mouse by a novel method of constant-flow infusion.
MedLine Citation:
PMID:  20861483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To characterize a technique that concurrently assesses all aqueous humor hydrodynamic parameters in mouse eyes.
METHODS: Mouse outflow facility (C) was determined by multiple flow-rate infusion and episcleral venous pressure (Pe) measured by manometry. The animals were then euthanatized, eliminating aqueous formation rate (Fin) and Pe. C was determined again (C(dead)) while uveoscleral outflow (Fu(dead)) and Fin were deduced. To assess whether Fu(dead) would remain the same as Fu(live), the animals were perfused with FITC-dextran and Fu determined. The effects of IOP-lowering drugs on the parameters of aqueous hydrodynamics were also evaluated.
RESULTS: Under the conditions tested, Fu(live) (0.012 ± 0.003 μL/min) was not different from Fu(dead) (0.015 ± 0.003 μL/min; P = 0.472). In anesthetized mice, IOP = 11.4 ± 0.2 mm Hg (mean ± SEM, n = 8), C = 0.018 ± 0.0006 μL/min/mm Hg, Pe = 5.4 ± 0.2 mm Hg, Fin = 0.14 ± 0.0007 μL/min, and Fu = 0.029 ± 0.005 μL/min. C(dead) was not different from C (P = 0.317). Latanoprost reduced IOP by increasing C by 0.009 ± 0.0003 μL/min/mm Hg (P < 0.001), without affecting Fin or Fu. Betaxolol reduced Fin by 0.075 ± 0.021 μL/min (P = 0.009). Brimonidine increased C by 0.005 ± 0.0005 μL/min/mm Hg (P < 0.001) and Fu by 0.013 ± 0.003 μL/min (P = 0.007).
CONCLUSIONS: In this study, a unique technique was developed to concurrently assess IOP, C, Pe, Fin, and Fu in the mouse eye. This experimental approach should be useful to evaluate effects of pharmacologic agents or genetic manipulations on aqueous humor dynamics in mice and other animal models.
Authors:
J Cameron Millar; Abbot F Clark; Iok-Hou Pang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-02-03
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  52     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-04     Completed Date:  2011-04-01     Revised Date:  2013-01-09    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  685-94     Citation Subset:  IM    
Affiliation:
Alcon Research, Ltd., Fort Worth, Texas 76134-2099, USA. cameron.millar@alconlabs.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Antihypertensive Agents / administration & dosage*
Aqueous Humor / metabolism*
Betaxolol / administration & dosage
Coloring Agents / diagnostic use
Dextrans / diagnostic use
Fluorescein-5-isothiocyanate / analogs & derivatives,  diagnostic use
Intraocular Pressure / drug effects,  physiology*
Male
Manometry
Mice
Mice, Inbred BALB C
Prostaglandins F, Synthetic / administration & dosage
Quinoxalines / administration & dosage
Sclera / metabolism*
Tonometry, Ocular
Trabecular Meshwork / metabolism*
Uvea / metabolism*
Venous Pressure
Chemical
Reg. No./Substance:
0/Antihypertensive Agents; 0/Coloring Agents; 0/Prostaglandins F, Synthetic; 0/Quinoxalines; 0/fluorescein isothiocyanate dextran; 130209-82-4/latanoprost; 3326-32-7/Fluorescein-5-isothiocyanate; 59803-98-4/brimonidine; 63659-18-7/Betaxolol; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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