| Assessment of apoptosis by immunohistochemistry to active caspase-3, active caspase-7, or cleaved PARP in monolayer cells and spheroid and subcutaneous xenografts of human carcinoma. | |
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MedLine Citation:
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PMID: 19029405 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immunohistochemistry to active caspase-3, recently recommended for apoptosis detection, is inappropriate to detect apoptosis involving caspase-7. Cleavage of poly-ADP-ribose polymerase 1 (PARP-1), a major substrate of both caspases, is a valuable marker of apoptosis. Apoptosis evaluation induced in vitro either by paclitaxel or by photodynamic treatment (PDT) with Foscan in HT29 or KB monolayer cells and HT29 spheroids yielded a close percentage of labeled cells whatever the antibody used, whereas in control specimens, cleaved PARP (c-PARP) immunostaining failed to detect apoptosis as efficiently as active caspase-3 or -7 immunostaining. Studies in MDA-MB231 monolayer cells and HT29 xenografts either subjected or not subjected to Foscan-PDT resulted in a significant higher number of active caspase-3-labeled cells, although immunofluorescence analysis showed c-PARP and active caspase-3 perfectly colocalized in tumors. A restricted expression of c-PARP was obvious in the greater part of caspase-3 expressing cells from control tumor, whereas photosensitized tumors showed a higher number of cells expressing large fluorescent spots from both active caspase-3 and c-PARP. These results support the assumption that c-PARP expression was dependent on treatment-induced apoptosis. The absence of caspase-7 activation in some caspase-3-expressing cells undergoing Foscan-PDT shows the relevance of using antibodies that can discriminate caspase-dependent apoptotic pathways. |
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Authors:
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Aude Bressenot; Sophie Marchal; Lina Bezdetnaya; Julie Garrier; François Guillemin; François Plénat |
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Publication Detail:
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Type: Journal Article Date: 2008-11-24 |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 57 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-17 Completed Date: 2009-04-08 Revised Date: 2010-09-23 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: United States |
Other Details:
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Languages: eng Pagination: 289-300 Citation Subset: IM |
Affiliation:
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Service d'Anatomie et de Cytologie Pathologiques, Hôpital de Brabois, Centre Hospitalier Régional et Universitaire Nancy, Vandoeuvre-lès-Nancy, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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metabolism,
pathology* Animals Antineoplastic Agents / pharmacology Apoptosis* Caspase 3 / metabolism* Caspase 7 / metabolism* Colonic Neoplasms / metabolism, pathology* Humans Immunohistochemistry Mesoporphyrins / pharmacology Mice Mice, Nude Neoplasm Transplantation Paclitaxel / pharmacology Poly(ADP-ribose) Polymerases / metabolism* Spheroids, Cellular / metabolism* Transplantation, Heterologous Triazenes / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Mesoporphyrins; 0/Triazenes; 0/temoporfin; 33069-62-4/Paclitaxel; 7227-91-0/1-phenyl-3,3-dimethyltriazene; EC 2.4.2.30/PARP1 protein, human; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 7 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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