| Assessment of apoptosis by immunohistochemical markers compared to cellular morphology in ex vivo-stressed colonic mucosa. | |
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MedLine Citation:
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PMID: 15684335 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptosis competence is central to the prevention of cancer. Frequency of apoptotic cells, after a sample of colonic tissue is stressed, can be used to gauge apoptosis competence and, thus, possible susceptibility to colon cancer. The gold standard for assessment of apoptosis is morphological evaluation, but this requires an experienced microscopist. Easier-to-use immunohistochemical markers of apoptosis, applicable in archived paraffin-embedded tissue, have been commercially developed. Potentially useful apoptosis markers include cleaved cytokeratin-18 (c-CK18), cleaved caspase-3 (c-cas-3), cleaved lamin A (c-lam-A), phosphorylated histone H2AX (gammaH2AX), cleaved poly(ADP ribose) polymerase (c-PARP), and translocation of apoptosis-inducing factor (AIF). When tissue samples from freshly resected colon segments were challenged ex vivo with the bile acid deoxycholate, approximately 50% of goblet cells became apoptotic by morphologic criteria. This high level of morphologic apoptosis allowed quantitative comparison with the usefulness and specificity of immunohistochemical markers of apoptosis. The antibody to c-CK18 was almost as useful and about as specific as morphology for identifying apoptotic colonic epithelial cells. Antibodies to c-cas-3, c-lam-A, and gammaH2AX, though specific for apoptotic cells, were less useful. The antibody to c-PARP, though specific for apoptotic cells, had low usefulness, and the antibody to AIF was relatively nonspecific, under our conditions. |
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Authors:
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Hana Holubec; Claire M Payne; Harris Bernstein; Katerina Dvorakova; Carol Bernstein; Caroline N Waltmire; James A Warneke; Harinder Garewal |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 53 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-02-01 Completed Date: 2005-03-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: United States |
Other Details:
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Languages: eng Pagination: 229-35 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Biological Markers / metabolism Colon / metabolism, pathology* Colonic Neoplasms / pathology Deoxycholic Acid / pharmacology Humans Immunohistochemistry Intestinal Mucosa / metabolism, pathology* |
| Grant Support | |
ID/Acronym/Agency:
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P01 CA-72008/CA/NCI NIH HHS; P50 CA-95060/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 83-44-3/Deoxycholic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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