| Assessment of angiogenesis in osseointegration of a silica-collagen biomaterial using 3D-nano-CT. | |
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MedLine Citation:
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PMID: 21723963 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bony integration of biomaterials is a complex process in which angiogenesis plays a crucial role. We evaluated micro- and nano-CT imaging to demonstrate and quantify neovascularization in bony integration of a biomaterial and to give an image based estimation for the needed resolution for imaging angiogenesis in an animal model of femora defect healing. In 8 rats 5mm full-size defects were created at the left femur that was filled with silica-collagen bone substitute material and internally fixed with plate osteosynthesis. After 6 weeks the femora were infused in situ with Microfil, harvested and scanned for micro-CT (9 μm)(3) and nano-CT (3 μm)(3) imaging. Using those 3D images, the newly formed blood vessels in the area of the biomaterial were assessed and the total vascular volume fraction, the volume of the bone substitute material and the volume of the bone defect were quantitatively characterized. Results were complemented by histology. Differences were statistically assessed using (ANOVA). High-resolution nano-CT demonstrated new blood vessel formation surrounding the biomaterial in all animals at capillary level. Immunohistochemistry confirmed the newly formed blood vessels surrounding the bone substitute material. The mean vascular volume fraction (VVF) around the implant was calculated to be 3.01 ± 0.4%. The VVF was inversely correlated with the volume of the bone substitute material (r=0.8) but not with the dimension of the fracture zone (r=0.3). Nano-CT imaging is feasible for quantitative analysis of angiogenesis during bony integration of biomaterials and a promising tool in this context for the future. |
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Authors:
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Volker Alt; Daniela Vera Kögelmaier; Katrin S Lips; Vera Witt; Sabine Pacholke; Christian Heiss; Marian Kampschulte; Sascha Heinemann; Thomas Hanke; Ulrich Thormann; Reinhard Schnettler; Alexander C Langheinrich |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-06-23 |
Journal Detail:
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Title: Acta biomaterialia Volume: 7 ISSN: 1878-7568 ISO Abbreviation: Acta Biomater Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-08-29 Completed Date: 2011-12-14 Revised Date: 2012-01-09 |
Medline Journal Info:
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Nlm Unique ID: 101233144 Medline TA: Acta Biomater Country: England |
Other Details:
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Languages: eng Pagination: 3773-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Trauma Surgery, Justus-Liebig-University Giessen, Germany. volker.alt@chiru.med.uni-giessen.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biocompatible Materials Bone Substitutes / pharmacology Collagen / pharmacology* Fractures, Bone / pathology, radiography Immunohistochemistry Nanotechnology / methods* Neovascularization, Physiologic / drug effects* Osseointegration / drug effects* Perfusion Rats Silicon Dioxide / pharmacology* Tomography, X-Ray Computed / methods* |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Bone Substitutes; 7631-86-9/Silicon Dioxide; 9007-34-5/Collagen |
| Comments/Corrections | |
Erratum In:
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Acta Biomater. 2012 Jan;8(1):457 Note: Thormann, Ulrich [added] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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