Document Detail


Assessment of Populus wood chemistry following the introduction of a Bt toxin gene.
MedLine Citation:
PMID:  16452069     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Unintended changes in plant physiology, anatomy and metabolism as a result of genetic engineering are a concern as more transgenic plants are commercially deployed in the ecosystem. We compared the cell wall chemical composition of three Populus lines (Populus trichocarpa Torr. & A. Gray x Populus trichocarpa Bartr. ex Marsh., Populus trichocarpa x Populus nigra L. and Populus deltoides x Populus nigra) genetically modified to express the Cry3A or Cry3B2 protein of Bacillus thuringiensis (Bt) with the cell wall chemistry of non-transformed isogenic control lines. Three genetically modified clones, each represented by 10 independent transgenic lines, were analyzed by pyrolysis molecular beam mass spectrometry, gas chromatography/mass spectrometry and traditional wet chemical analytical methods to assess changes in cell wall composition. Based on the outcome of these techniques, there were no comprehensive differences in chemical composition between the transgenic and control lines for any of the studied clones.
Authors:
Mark F Davis; Gerald A Tuskan; Peggy Payne; Timothy J Tschaplinski; Richard Meilan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Tree physiology     Volume:  26     ISSN:  0829-318X     ISO Abbreviation:  Tree Physiol.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-02-02     Completed Date:  2007-06-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100955338     Medline TA:  Tree Physiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  557-64     Citation Subset:  IM    
Affiliation:
National Center for Bioenergy, National Renewable Energy Laboratory, Golden, CO 80401, USA. mark_davis@nrel.gov
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MeSH Terms
Descriptor/Qualifier:
Bacillus thuringiensis / genetics*
Bacterial Toxins / genetics*
Cell Wall / metabolism
Gas Chromatography-Mass Spectrometry
Genetic Vectors
Mass Spectrometry
Plants, Genetically Modified
Populus / genetics*,  metabolism
Wood / chemistry*,  metabolism
Chemical
Reg. No./Substance:
0/Bacterial Toxins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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