Document Detail

Assessment of the photoprotection properties of sunscreens by chromatographic measurement of DNA damage in skin explants.
MedLine Citation:
PMID:  21091484     Owner:  NLM     Status:  In-Process    
Evaluation of the photoprotection provided by sunscreens is performed either through the induction of erythema and expressed as the sun protection factor (SPF), or by the UVA-mediated persistent pigment darkening (PPD). None of these two endpoints has a link with skin cancer, the most deleterious consequence of excess exposure to solar UV radiation. We thus set up a complementary approach to evaluate the protection provided by sunscreens to the genome of human skin. This is based on the quantification of the thymine cyclobutane dimer (TT-CPD), the main DNA lesion induced by both UVB and UVA radiations. Irradiations were performed ex vivo on human skin explants and the level of TT-CPD in DNA was determined by HPLC associated with tandem mass spectrometry. The technique was first optimized and validated with three standard sunscreens. The study was then extended to the evaluation of a commercial high SPF sunscreen exhibiting efficient UVA photoprotection. The DNA protecting factor was found to reflect the ratio between UVB and UVA photoprotection, although the absolute values of the genomic protection were, as a general trend, lower than either SPF or PPD. These data show the usefulness of the proposed approach for the evaluation of the genoprotection afforded by sunscreens.
Stéphane Mouret; Patrick Bogdanowicz; Marie-José Haure; Nathalie Castex-Rizzi; Jean Cadet; Alain Favier; Thierry Douki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-19
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  87     ISSN:  1751-1097     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:    2011 Jan-Feb
Date Detail:
Created Date:  2010-12-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  109-16     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Photochemistry and Photobiology © 2010 The American Society of Photobiology.
CEA, INAC, SCIB, UJF & CNRS, LCIB (UMR_E 3 CEA-UJF and FRE 3200), Laboratoire Lésions des Acides Nucléiques, Grenoble Cedex, France.
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