Document Detail


Assessment of P-glycoprotein-dependent drug transport in isolated rat hepatocytes using rhodamine 123.
MedLine Citation:
PMID:  7905358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To test the activity of P-170 glycoprotein in isolated hepatocytes, a method has been developed employing the fluorescent dye rhodamine 123 (R-123). The uptake of R-123 by both freshly isolated and 4-hr-plated hepatocytes depends on dye concentration, time of incubation, and cell number. The efflux of R-123 from cells is inhibited by sodium azide and by verapamil. In standard conditions the efficiency of efflux of R-123 from cells correlates with the relative amount of immunoblottable glycoprotein. The method has been applied to detection of P-170 activity in hepatocytes from animals of different ages as well as from carcinogen-treated animals. The proposed assay appears a simple and adequate tool for the functional assessment of multidrug transporter in liver.
Authors:
E Chieli; E Santoni Rugiu; F Cervelli; A Paolicchi; R Tongiani
Related Documents :
12134948 - Characterization of the efflux transporter(s) responsible for restricting intestinal mu...
23088378 - Nervous network for lower urinary tract function.
19300998 - Influence of multidrug resistance on (18)f-fch cellular uptake in a glioblastoma model.
19496068 - Expression of multidrug resistance proteins in prostate cancer is related with cell sen...
19968988 - Myosin 1g (myo1g) is a haematopoietic specific myosin that localises to the plasma memb...
21784698 - Molecular mechanism of aspergillus fumigatus adherence to host constituents.
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology and toxicology     Volume:  9     ISSN:  0742-2091     ISO Abbreviation:  Cell Biol. Toxicol.     Publication Date:    1993 Jul-Sep
Date Detail:
Created Date:  1994-03-04     Completed Date:  1994-03-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506639     Medline TA:  Cell Biol Toxicol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  235-41     Citation Subset:  IM    
Affiliation:
Dipartimento de Biomedicina Sperimentale, Università di Pisa, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Azides / pharmacology
Biological Transport, Active / drug effects
Carrier Proteins / metabolism*
Cell Membrane / drug effects,  metabolism
Drug Resistance
Fluorescent Dyes / pharmacokinetics
Liver / drug effects,  metabolism*
Male
Membrane Glycoproteins / metabolism*
P-Glycoprotein
Rats
Rats, Sprague-Dawley
Rhodamine 123
Rhodamines / pharmacokinetics
Sodium Azide
Verapamil / pharmacology
Chemical
Reg. No./Substance:
0/Azides; 0/Carrier Proteins; 0/Fluorescent Dyes; 0/Membrane Glycoproteins; 0/P-Glycoprotein; 0/Rhodamines; 26628-22-8/Sodium Azide; 52-53-9/Verapamil; 62669-70-9/Rhodamine 123

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Endogenous nitric oxide inhibits NMDA- and kainate- responses by a negative feedback system in rat h...
Next Document:  Sedatives, tranquilizers, and stimulants.