Document Detail


Assessment of genetic damage in buccal epithelium cells of painters: micronucleus, nuclear changes, and repair index.
MedLine Citation:
PMID:  20218900     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The micronucleus (MN) assay in exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans exposed to occupational and environmental agents. The MN test is used as an indicator of genotoxic exposition, since it is associated with chromosome aberrations. An increased mutation rate in oral squamous cells, which is indicated by an increased MN frequency, is also related to the development of oral mucosa diseases, such as carcinomas. We evaluated MN frequencies and other nuclear changes (NCs), karyorrhexis (KR), karyolysis (KL), broken egg (BE), and binucleus in buccal mucosa cells of 60 painters (30 smokers and 30 nonsmokers) and 60 healthy control subjects (30 smoker and 30 nonsmoker). Microscopic observation of 3000 cells per individual was performed in both painters and control subjects. In the control group and the exposed group, for each person repair index (RI) was calculated via the following formula: (KR + KL)/(BE + MN). The results showed a statistically significant increase in the frequency of MN in buccal epithelial cells of the exposed group compared with the control group (p < 0.05). Smokers and nonsmokers differed with respect to the incidence of MN and NCs in all groups. In painters, RI was less than that in the control group. There was a significant difference between painters and the control group (p < 0.01) for RI. We believe that determination of RI in addition to NCs and the MN will present a new approach to genotoxicity studies of a population.
Authors:
Ayla Celik; Song?l Budak Diler; Dilek Eke
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  DNA and cell biology     Volume:  29     ISSN:  1557-7430     ISO Abbreviation:  DNA Cell Biol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-18     Completed Date:  2010-07-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9004522     Medline TA:  DNA Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  277-84     Citation Subset:  IM    
Affiliation:
Department of Biology, Faculty of Science and Letters, Mersin University, Mersin, Turkey. a.celik@mersin.edu.tr
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MeSH Terms
Descriptor/Qualifier:
Adult
Age Factors
Cell Count
Cell Nucleus / drug effects,  genetics*
Humans
Male
Micronucleus Tests / methods*
Mouth Mucosa / drug effects,  metabolism*,  pathology*
Mutagens / toxicity
Occupational Exposure / adverse effects*
Paintings*
Smoking / genetics
Time Factors
Chemical
Reg. No./Substance:
0/Mutagens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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