Document Detail


Assessment of dental fluorosis in Mmp20 +/- mice.
MedLine Citation:
PMID:  21386097     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The molecular mechanisms that underlie dental fluorosis are poorly understood. The retention of enamel proteins hallmarking fluorotic enamel may result from impaired hydrolysis and/or removal of enamel proteins. Previous studies have suggested that partial inhibition of Mmp20 expression is involved in the etiology of dental fluorosis. Here we ask if mice expressing only one functional Mmp20 allele are more susceptible to fluorosis. We demonstrate that Mmp20 (+/-) mice express approximately half the amount of MMP20 as do wild-type mice. The Mmp20 heterozygous mice have normal-appearing enamel, with Vickers microhardness values similar to those of wild-type control enamel. Therefore, reduced MMP20 expression is not solely responsible for dental fluorosis. With 50-ppm-fluoride (F(-)) treatment ad libitum, the Mmp20 (+/-) mice had F(-) tissue levels similar to those of Mmp20 (+/+) mice. No significant difference in enamel hardness was observed between the F(-)-treated heterozygous and wild-type mice. Interestingly, we did find a small but significant difference in quantitative fluorescence between these two groups, which may be attributable to slightly higher protein content in the Mmp20 (+/-) mouse enamel. We conclude that MMP20 plays a nominal role in dental enamel fluorosis.
Authors:
R Sharma; C E Tye; A Arun; D MacDonald; A Chatterjee; T Abrazinski; E T Everett; G M Whitford; J D Bartlett
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-03-08
Journal Detail:
Title:  Journal of dental research     Volume:  90     ISSN:  1544-0591     ISO Abbreviation:  J. Dent. Res.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-11     Completed Date:  2011-07-18     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  0354343     Medline TA:  J Dent Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  788-92     Citation Subset:  D; IM    
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MeSH Terms
Descriptor/Qualifier:
Amelogenesis
Animals
Dental Enamel / chemistry,  enzymology
Dental Enamel Proteins / metabolism
Enamel Organ / enzymology
Fluorescence
Fluorides / adverse effects*
Fluorosis, Dental / enzymology*,  etiology*,  genetics
Gene Expression Regulation, Developmental / drug effects*
Hardness
Heterozygote
Homozygote
Matrix Metalloproteinase 20 / analysis,  biosynthesis*,  genetics
Mice
Mice, Inbred C57BL
Grant Support
ID/Acronym/Agency:
DE018104/DE/NIDCR NIH HHS; DE018106/DE/NIDCR NIH HHS; DE16276/DE/NIDCR NIH HHS; R01 DE016276/DE/NIDCR NIH HHS; R01 DE016276-04S1/DE/NIDCR NIH HHS; R01 DE016276-05/DE/NIDCR NIH HHS; R01 DE018104/DE/NIDCR NIH HHS; R01 DE018106/DE/NIDCR NIH HHS; R01 DE018106-02/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Dental Enamel Proteins; EC 3.4.24.-/Matrix Metalloproteinase 20; Q80VPU408O/Fluorides
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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