Document Detail


Assessment of the AmnioStat-FLM immunoagglutination test for phosphatidylglycerol in amniotic fluid.
MedLine Citation:
PMID:  3719984     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One hundred and eight amniotic fluids were assayed by the AmnioStat-FLM (A-FLM) immunological agglutination test for phosphatidylglycerol (PG) and simultaneously measured enzymatically for PG content. Of 52 amniotic fluids found to be PG negative by the A-FLM method, all had enzymatic PG concentrations less than or equal to 1.5 mumol/l. Conversely, of 56 amniotic fluids judged to be either PG positive or weak positive, all but five had enzymatic PG concentrations greater than 1.5 mumol/l. The sensitivity of the A-FLM assay employed clinically for predicting foetal lung maturity was 89% and the specificity was 100%. The overall predictive accuracy of the test could be improved by providing controls at lower, more appropriate PG concentrations. Ninety-one fluids analysed by the A-FLM kit were subsequently tested for the presence of PG by two-dimensional thin-layer chromatography (2D TLC). A 94%-concordance between the methods was found.
Authors:
J Farquharson; E C Jamieson; E Berry; R Buchanan; R W Logan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  156     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-07-25     Completed Date:  1986-07-25     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  271-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Agglutination Tests / instrumentation
Amniotic Fluid / analysis*
Chromatography, Thin Layer
Female
Fetal Organ Maturity
Gestational Age
Humans
Lung / embryology
Phosphatidylglycerols / analysis*
Pregnancy
Chemical
Reg. No./Substance:
0/Phosphatidylglycerols

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