Document Detail


Assessing the role of hematopoietic plasticity for endothelial and hepatocyte development by non-invasive lineage tracing.
MedLine Citation:
PMID:  15576407     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hematopoietic cells have been reported to convert into a number of non-hematopoietic cells types after transplantation/injury. Here, we have used a lineage tracing approach to determine whether hematopoietic plasticity is relevant for the normal development of hepatocytes and endothelial cells, both of which develop in close association with blood cells. Two mouse models were analyzed: vav ancestry mice, in which essentially all hematopoietic cells, including stem cells, irreversibly express yellow fluorescent protein (YFP); and lysozyme ancestry mice, in which all macrophages, as well as a small subset of all other non-myeloid hematopoietic cells, are labeled. Both lines were found to contain YFP+ hepatocytes at similar frequencies, indicating that macrophage to hepatocyte contributions occur in unperturbed mice. However, the YFP+ hepatocytes never formed clusters larger than three cells, suggesting a postnatal origin. In addition, the frequency of these cells was very low (approximately 1 in 75,000) and only increased two- to threefold after acute liver injury. Analysis of the two mouse models revealed no evidence for a hematopoietic origin of endothelial cells, showing that definitive HSCs do not function as hemangioblasts during normal development. Using endothelial cells and hepatocytes as paradigms, our study indicates that hematopoietic cells are tightly restricted in their differentiation potential during mouse embryo development and that hematopoietic plasticity plays at best a minor role in adult organ maintenance and regeneration.
Authors:
Matthias Stadtfeld; Thomas Graf
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2004-12-02
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  132     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-13     Completed Date:  2005-03-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  203-13     Citation Subset:  IM    
Affiliation:
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / metabolism
Bone Marrow Cells
Cell Lineage
Cell Separation
Endothelium, Vascular / cytology*,  metabolism
Flow Cytometry
Hematopoietic Stem Cells / cytology*
Hepatocytes / cytology*,  metabolism
Lac Operon
Liver / cytology,  embryology,  pathology
Luminescent Proteins / metabolism
Macrophages / metabolism
Mice
Microscopy, Fluorescence
Models, Biological
Muramidase / metabolism
Plasmids / metabolism
Polymerase Chain Reaction
Regeneration
Time Factors
Grant Support
ID/Acronym/Agency:
R01 NS43881-01/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Luminescent Proteins; 0/yellow fluorescent protein, Bacteria; EC 3.2.1.17/Muramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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