Document Detail


Assessing recruitment of lung diffusing capacity in exercising guinea pigs with a rebreathing technique.
MedLine Citation:
PMID:  18483171     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Noninvasive techniques for assessing cardiopulmonary function in small animals are limited. We previously developed a rebreathing technique for measuring lung volume, pulmonary blood flow, diffusing capacity for carbon monoxide (Dl(CO)) and its components, membrane diffusing capacity (Dm(CO)) and pulmonary capillary blood volume (Vc), and septal volume, in conscious nonsedated guinea pigs at rest. Now we have extended this technique to study guinea pigs during voluntary treadmill exercise with a sealed respiratory mask attached to a body vest and a test gas mixture containing 0.5% SF(6) or Ne, 0.3% CO, and 0.8% C(2)H(2) in 40% or 98% O(2). From rest to exercise, O(2) uptake increased from 12.7 to 25.5 ml x min(-1) x kg(-1) while pulmonary blood flow increased from 123 to 239 ml/kg. The measured Dl(CO), Dm(CO), and Vc increased linearly with respect to pulmonary blood flow as expected from alveolar microvascular recruitment; body mass-specific relationships were consistent with those in healthy human subjects and dogs studied with a similar technique. The results show that 1) cardiopulmonary interactions from rest to exercise can be measured noninvasively in guinea pigs, 2) guinea pigs exhibit patterns of exercise response and alveolar microvascular recruitment similar to those of larger species, and 3) the rebreathing technique is widely applicable to human ( approximately 70 kg), dog (20-30 kg), and guinea pig (1-1.5 kg). In theory, this technique can be extended to even smaller animals provided that species-specific technical hurdles can be overcome.
Authors:
Cuneyt Yilmaz; D Merrill Dane; Connie C W Hsia
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-05-15
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  105     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-21     Completed Date:  2008-09-03     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  316-21     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390-9034, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Volume / physiology
Body Temperature / physiology
Body Weight / physiology
Capillaries / physiology
Carbon Monoxide / metabolism
Guinea Pigs
Male
Oxygen Consumption / physiology
Perfusion
Physical Conditioning, Animal / physiology*
Pulmonary Circulation / physiology
Pulmonary Diffusing Capacity / methods*,  physiology*
Pulmonary Gas Exchange / physiology
Grant Support
ID/Acronym/Agency:
HL-045716/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
630-08-0/Carbon Monoxide
Comments/Corrections

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