Document Detail


Assessing problems of organic hydroperoxide-induced chemiluminescence related to biological/medical samples.
MedLine Citation:
PMID:  15560941     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The supernatants obtained from mammalian tissue homogenates, blood serum or other body fluids, and supplemented with organic hydroperoxide show a multiplicity of light-emitting reactions. The low-level chemiluminescence of biological samples denotes that after hydroperoxide-induction electronically excited species are produced. The light-emission measured by photomultipliers is related to (i) the geometrical location of the induced sample, (ii) light gathering capacity of the phototubes, and (iii) both the quantity and concentration of samples and reactants. The fewest blood contamination of biological samples leads to an increased photoemission. The very high sensitivity of light detectors requires stable measuring and sampling conditions and peculiarly cautious evaluation of the reactions.
Authors:
B Török
Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of biochemical and biophysical methods     Volume:  61     ISSN:  0165-022X     ISO Abbreviation:  J. Biochem. Biophys. Methods     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-11-24     Completed Date:  2005-04-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7907378     Medline TA:  J Biochem Biophys Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  247-51     Citation Subset:  IM    
Affiliation:
Bioanalytical Institute, Medical Faculty, University of Pécs, Szigeti út 12, 7624, Pécs, Hungary. bela.torok@aok.pte.hu
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MeSH Terms
Descriptor/Qualifier:
Artifacts*
Chemiluminescent Measurements / methods*
Hydrogen Peroxide / analysis*,  pharmacology*
Reactive Oxygen Species / analysis*,  metabolism*
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Reactive Oxygen Species; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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