| Assessing the potential toxic effect of one persistent organic pollutant: Non-covalent interaction of dicofol with the enzyme trypsin. | |
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MedLine Citation:
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PMID: 22261109 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Because of the widespread concern that persistent organic pollutants (POPs) may be adversely affecting the health of humans, reliable assessing their toxic effects is urgently needed. We selectively study the interaction between dicofol (DCF) and trypsin by steady state and time resolved fluorescence quenching measurements and UV-visible absorption spectroscopy under physiological conditions as well as applying molecular docking method to establish the interaction model. The fluorescence results indicate DCF can spontaneously form a complex with trypsin mainly by hydrogen bond with only one binding site, which had been validated in molecular docking. The conformational change of trypsin was proved by UV-visible absorption and synchronous fluorescence spectroscopy indicating a red shift of carbonyl absorption peak. All the results indicated DCF had potential toxic effects on both the structure and activity of the enzyme trypsin and the effects enhanced with the increasing concentration of DCF. |
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Authors:
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Ying Liu; Rongxiang Cao; Pengfei Qin; Rutao Liu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-31 |
Journal Detail:
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Title: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Volume: 89C ISSN: 1873-3557 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2012-1-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9602533 Medline TA: Spectrochim Acta A Mol Biomol Spectrosc Country: - |
Other Details:
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Languages: ENG Pagination: 210-215 Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, America CRC for Environment & Health Shandong Province, Shandong University, 27# Shanda South Road, Jinan 250100, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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