Document Detail


Assessing mitochondrial dysfunction in cells.
MedLine Citation:
PMID:  21726199     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Assessing mitochondrial dysfunction requires definition of the dysfunction to be investigated. Usually, it is the ability of the mitochondria to make ATP appropriately in response to energy demands. Where other functions are of interest, tailored solutions are required. Dysfunction can be assessed in isolated mitochondria, in cells or in vivo, with different balances between precise experimental control and physiological relevance. There are many methods to measure mitochondrial function and dysfunction in these systems. Generally, measurements of fluxes give more information about the ability to make ATP than do measurements of intermediates and potentials. For isolated mitochondria, the best assay is mitochondrial respiratory control: the increase in respiration rate in response to ADP. For intact cells, the best assay is the equivalent measurement of cell respiratory control, which reports the rate of ATP production, the proton leak rate, the coupling efficiency, the maximum respiratory rate, the respiratory control ratio and the spare respiratory capacity. Measurements of membrane potential provide useful additional information. Measurement of both respiration and potential during appropriate titrations enables the identification of the primary sites of effectors and the distribution of control, allowing deeper quantitative analyses. Many other measurements in current use can be more problematic, as discussed in the present review.
Authors:
Martin D Brand; David G Nicholls
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  The Biochemical journal     Volume:  435     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-07-05     Completed Date:  2011-09-16     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  297-312     Citation Subset:  IM    
Affiliation:
Buck Institute for Research on Aging, 8001 Redwood Blvd, Novato, CA 94945, USA. mbrand@buckinstitute.org
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells / metabolism*,  pathology,  ultrastructure*
Clinical Laboratory Techniques*
Electron Transport / physiology
Energy Metabolism
Humans
Mitochondria / metabolism,  pathology,  physiology*
Mitochondrial Diseases / diagnosis*,  metabolism,  pathology,  physiopathology
Models, Biological
Protons
Grant Support
ID/Acronym/Agency:
P01 AG025901/AG/NIA NIH HHS; P30 AG025708/AG/NIA NIH HHS; PL1 AG032118/AG/NIA NIH HHS; R01 AG033542/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Protons
Comments/Corrections
Erratum In:
Biochem J. 2011 Aug 1;437(3):575

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