Document Detail


Assessing hazelnut allergens by protein- and DNA-based approaches: LC-MS/MS, ELISA and real-time PCR.
MedLine Citation:
PMID:  24577577     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Hazelnut (Corylus avellana L.) is responsible for a significant part of the allergies related to nuts. Still, it is a very much appreciated nut and as consequence is widely used in all types of processed foods, such as chocolates. Correct food labelling is currently the most effective means of preventing the consumption of allergenic ingredients, namely hazelnut, by the sensitised/allergic individuals. Thus, to verify labelling compliance and to ensure allergic patient protection, the development of highly sensitive methodologies is of extreme importance. In this study, three major methodologies, namely enzyme-linked immunosorbent assays (ELISA), liquid chromatography coupled with mass spectrometry and real-time polymerase chain reaction, were evaluated for their performance regarding the detection of hazelnut allergens in model chocolates. The sandwich ELISA and respective antibodies were in-house developed and produced. With sensitivity levels of approximately 1 mg kg(-1) and limits of quantification of 50-100 mg kg(-1), all the performed methods were considered appropriate for the identification of hazelnut in complex foods such as chocolates. To our knowledge, this was the first successful attempt to develop and compare three independent approaches for the detection of allergens in foods.
Authors:
Joana Costa; Parisa Ansari; Isabel Mafra; M Beatriz P P Oliveira; Sabine Baumgartner
Related Documents :
12317907 - Chicano return migration to the southwest: an integrated human capital approach.
23618637 - Front-of-pack nutrition labelling systems: a missed opportunity?
24439347 - Traffic-light labels and choice architecture: promoting healthy food choices.
15590617 - The property 'instinct'.
18063017 - Continuous measurements of aerosol physical parameters at the mt. cimone gaw station (2...
10477057 - Heritability of hunger relationships with food intake in free-living humans.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-2-28
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  -     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2014 Feb 
Date Detail:
Created Date:  2014-2-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Application of liquid chromatography quadrupole time-of-flight mass spectrometry to the identificati...
Next Document:  Metabolomic profiling of mice urine and serum associated with trans-trans 2, 4-decadienal induced lu...