Document Detail


Assessing embryonic stem cell response to surface chemistry using plasma polymer gradients.
MedLine Citation:
PMID:  22326974     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The control of cell-material interactions is the key to a broad range of biomedical interactions. Gradient surfaces have recently been established as tools allowing the high-throughput screening and optimisation of these interactions. In this report, we show that plasma polymer gradients can reveal the subtle influence of surface chemistry on embryonic stem (ES) cell behaviour and probe the mechanisms by which this occurs. Lateral gradients of surface chemistry were generated by plasma polymerisation of diethyleneglycol dimethyl ether (DG) on top of a substrate coated with an acrylic acid (AA) plasma polymer using a tilted slide as a mask. Gradient surfaces were characterised by X-ray photoelectron spectroscopy, infrared microscopy mapping and profilometry. By changing the plasma polymerisation time, the gradient profile could be easily manipulated. To demonstrate the utility of these surfaces for the screening of cell-material interactions, we studied the response of mouse ES cells to these gradients and compared the performance of different plasma polymerisation times during gradient fabrication. We observed a strong correlation between surface chemistry and cell attachment, colony size and retention of stem cell markers. Cell adhesion and colony formation showed striking differences on gradients with different plasma polymer deposition time. Deposition time influenced the depth of the plasma film deposited and the relative position of surface functional group density on the substrate, but not the range of plasma-generated species.
Authors:
Frances J Harding; Lauren R Clements; Robert D Short; Helmut Thissen; Nicolas H Voelcker
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-8
Journal Detail:
Title:  Acta biomaterialia     Volume:  -     ISSN:  1878-7568     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233144     Medline TA:  Acta Biomater     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ltd.
Affiliation:
School of Chemical and Physical Sciences, Flinders University, Bedford Park SA 5042, Australia.
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