Document Detail


Assessing delivery and quantifying efficacy of small interfering ribonucleic acid therapeutics in the skin using a dual-axis confocal microscope.
MedLine Citation:
PMID:  20615029     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transgenic reporter mice and advances in imaging instrumentation are enabling real-time visualization of cellular mechanisms in living subjects and accelerating the development of novel therapies. Innovative confocal microscope designs are improving their utility for microscopic imaging of fluorescent reporters in living animals. We develop dual-axis confocal (DAC) microscopes for such in vivo studies and create mouse models where fluorescent proteins are expressed in the skin for the purpose of advancing skin therapeutics and transdermal delivery tools. Three-dimensional image volumes, through the different skin compartments of the epidermis and dermis, can be acquired in several seconds with the DAC microscope in living mice, and are comparable to histologic analyses of reporter protein expression patterns in skin sections. Intravital imaging with the DAC microscope further enables visualization of green fluorescent protein (GFP) reporter gene expression in the skin over time, and quantification of transdermal delivery of small interfering RNA (siRNA) and therapeutic efficacy. Visualization of transdermal delivery of nucleic acids will play an important role in the development of innovative strategies for treating skin pathologies.
Authors:
Hyejun Ra; Emilio Gonzalez-Gonzalez; Bryan R Smith; Sanjiv S Gambhir; Gordon S Kino; Olav Solgaard; Roger L Kaspar; Christopher H Contag
Related Documents :
17286699 - A simple technique of image analysis for specific nuclear immunolocalization of proteins.
24111079 - Comparison of logistic regression and neural network classifiers in the detection of ha...
8858859 - Application of confocal scanning laser microscopy for an automated nuclear grading of p...
23542939 - Biophotonics techniques for structural and functional imaging, in vivo.
23414629 - Assessment of acquisition protocols for routine imaging of y-90 using pet/ct.
19123569 - Microbeam-integrated multiphoton imaging system.
11079019 - Effect of image compression on telepathology. a randomized clinical trial.
2285079 - Pseudomaturity in the developmental line of object relations.
21128449 - Spiral scanning method for atomic force microscopy.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  15     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:    2010 May-Jun
Date Detail:
Created Date:  2010-07-09     Completed Date:  2010-10-29     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  036027     Citation Subset:  IM    
Affiliation:
Stanford University, Department of Electrical Engineering, Ginzton Laboratory, Stanford, California 94305, USA. hra@stanford.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Drug Delivery Systems*
Foot
Gene Expression / drug effects
Genes, Reporter
Green Fluorescent Proteins / administration & dosage,  chemistry,  genetics,  metabolism
Mice
Mice, Transgenic
Microscopy, Confocal / methods*
RNA, Small Interfering / administration & dosage*,  chemistry,  genetics
Skin
Grant Support
ID/Acronym/Agency:
R44 AR055881/AR/NIAMS NIH HHS; U54 CA105296/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Small Interfering; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inspired gas-induced vascular change in tumors with magnetic-resonance-guided near-infrared imaging:...
Next Document:  Quantitative polarized Raman spectroscopy in highly turbid bone tissue.