| Assessing delivery and quantifying efficacy of small interfering ribonucleic acid therapeutics in the skin using a dual-axis confocal microscope. | |
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MedLine Citation:
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PMID: 20615029 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transgenic reporter mice and advances in imaging instrumentation are enabling real-time visualization of cellular mechanisms in living subjects and accelerating the development of novel therapies. Innovative confocal microscope designs are improving their utility for microscopic imaging of fluorescent reporters in living animals. We develop dual-axis confocal (DAC) microscopes for such in vivo studies and create mouse models where fluorescent proteins are expressed in the skin for the purpose of advancing skin therapeutics and transdermal delivery tools. Three-dimensional image volumes, through the different skin compartments of the epidermis and dermis, can be acquired in several seconds with the DAC microscope in living mice, and are comparable to histologic analyses of reporter protein expression patterns in skin sections. Intravital imaging with the DAC microscope further enables visualization of green fluorescent protein (GFP) reporter gene expression in the skin over time, and quantification of transdermal delivery of small interfering RNA (siRNA) and therapeutic efficacy. Visualization of transdermal delivery of nucleic acids will play an important role in the development of innovative strategies for treating skin pathologies. |
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Authors:
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Hyejun Ra; Emilio Gonzalez-Gonzalez; Bryan R Smith; Sanjiv S Gambhir; Gordon S Kino; Olav Solgaard; Roger L Kaspar; Christopher H Contag |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical optics Volume: 15 ISSN: 1560-2281 ISO Abbreviation: J Biomed Opt Publication Date: 2010 May-Jun |
Date Detail:
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Created Date: 2010-07-09 Completed Date: 2010-10-29 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 9605853 Medline TA: J Biomed Opt Country: United States |
Other Details:
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Languages: eng Pagination: 036027 Citation Subset: IM |
Affiliation:
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Stanford University, Department of Electrical Engineering, Ginzton Laboratory, Stanford, California 94305, USA. hra@stanford.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Drug Delivery Systems* Foot Gene Expression / drug effects Genes, Reporter Green Fluorescent Proteins / administration & dosage, chemistry, genetics, metabolism Mice Mice, Transgenic Microscopy, Confocal / methods* RNA, Small Interfering / administration & dosage*, chemistry, genetics Skin |
| Grant Support | |
ID/Acronym/Agency:
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R44 AR055881/AR/NIAMS NIH HHS; U54 CA105296/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Small Interfering; 147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
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