Document Detail

Assessing delivery and quantifying efficacy of small interfering ribonucleic acid therapeutics in the skin using a dual-axis confocal microscope.
MedLine Citation:
PMID:  20615029     Owner:  NLM     Status:  MEDLINE    
Transgenic reporter mice and advances in imaging instrumentation are enabling real-time visualization of cellular mechanisms in living subjects and accelerating the development of novel therapies. Innovative confocal microscope designs are improving their utility for microscopic imaging of fluorescent reporters in living animals. We develop dual-axis confocal (DAC) microscopes for such in vivo studies and create mouse models where fluorescent proteins are expressed in the skin for the purpose of advancing skin therapeutics and transdermal delivery tools. Three-dimensional image volumes, through the different skin compartments of the epidermis and dermis, can be acquired in several seconds with the DAC microscope in living mice, and are comparable to histologic analyses of reporter protein expression patterns in skin sections. Intravital imaging with the DAC microscope further enables visualization of green fluorescent protein (GFP) reporter gene expression in the skin over time, and quantification of transdermal delivery of small interfering RNA (siRNA) and therapeutic efficacy. Visualization of transdermal delivery of nucleic acids will play an important role in the development of innovative strategies for treating skin pathologies.
Hyejun Ra; Emilio Gonzalez-Gonzalez; Bryan R Smith; Sanjiv S Gambhir; Gordon S Kino; Olav Solgaard; Roger L Kaspar; Christopher H Contag
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  15     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:    2010 May-Jun
Date Detail:
Created Date:  2010-07-09     Completed Date:  2010-10-29     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  036027     Citation Subset:  IM    
Stanford University, Department of Electrical Engineering, Ginzton Laboratory, Stanford, California 94305, USA.
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MeSH Terms
Drug Delivery Systems*
Gene Expression / drug effects
Genes, Reporter
Green Fluorescent Proteins / administration & dosage,  chemistry,  genetics,  metabolism
Mice, Transgenic
Microscopy, Confocal / methods*
RNA, Small Interfering / administration & dosage*,  chemistry,  genetics
Grant Support
Reg. No./Substance:
0/RNA, Small Interfering; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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