| Assessing crystallization droplets using birefringence. | |
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MedLine Citation:
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PMID: 15039558 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this paper, the detection of crystalline elements in protein crystallization droplets containing precipitate is illustrated using the rotating-polarizer microscope technique. The sensitivity of this automated birefringence technique enables the detection of microcrystals in a precipitate that appears to be amorphous using traditional methods of inspection. The technique is illustrated with lysozyme and glucose isomerase. Glucose isomerase microcrystals were used successfully for seeding experiments and the conditions of both of the systems were refined to produce crystals suitable for X-ray analysis. The results are relevant to the field of high-throughput crystallography as an automated crystal-detection method as well as being a useful tool for detailed precipitate analysis. |
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Authors:
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A Echalier; R L Glazer; V Fülöp; M A Geday |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-03-23 |
Journal Detail:
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Title: Acta crystallographica. Section D, Biological crystallography Volume: 60 ISSN: 0907-4449 ISO Abbreviation: Acta Crystallogr. D Biol. Crystallogr. Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-03-24 Completed Date: 2004-12-07 Revised Date: 2007-07-24 |
Medline Journal Info:
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Nlm Unique ID: 9305878 Medline TA: Acta Crystallogr D Biol Crystallogr Country: Denmark |
Other Details:
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Languages: eng Pagination: 696-702 Citation Subset: IM |
Affiliation:
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University of Warwick, Department of Biological Sciences, Gibbet Hill Road, Coventry CV4 7AL, England. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aldose-Ketose Isomerases
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chemistry Birefringence Crystallization* / methods Crystallography, X-Ray Muramidase / chemistry Proteins / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Proteins; EC 3.2.1.17/Muramidase; EC 5.3.1/Aldose-Ketose Isomerases; EC 5.3.1.5/xylose isomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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