Document Detail


Assessing the contamination potential of freshly extracted Escherichia coli biofilm cells by impedancemetry.
MedLine Citation:
PMID:  16897295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Planktonic bacteria passing to a sessile state during the formation of a biofilm undergo many gene expression and phenotypic changes. These transformations require a significant time to establish. Inversely, cells extracted from a biofilm should also require a significant time before acquiring the same physiological characteristics as planktonic cells. Relatively few studies have addressed the kinetics of this inverse transformation process. We tested one aspect, namely, the contamination potential of freshly extracted Escherichia coli biofilm cells, precultured in a synthetic medium, in a rich liquid growth medium. We compared the time between inoculation and the beginning of the growth phase of freshly extracted biofilm cells, and suspended exponential and suspended stationary phase cells precultured in the same synthetic medium. Unexpectedly, the lag time for the extracted biofilm cells was the same as the lag time of the suspended exponential phase cells and significantly less than the lag time of the suspended stationary phase cells. The lag times were determined by an impedance technique. Cells extracted from biofilms, i.e., biofilms formed in canalizations and broken up by hydrodynamic forces, are an important source of contamination. Our work shows, in the case of E. coli, the high potential of freshly extracted biofilm cells to reinfect a new medium.
Authors:
R Caubet; F Pedarros-Caubet; Y Quataert; A Lescure; J M Moreau; W J Ellison
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2006-08-08
Journal Detail:
Title:  Microbial ecology     Volume:  52     ISSN:  0095-3628     ISO Abbreviation:  Microb. Ecol.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-09-12     Completed Date:  2006-11-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7500663     Medline TA:  Microb Ecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  239-43     Citation Subset:  IM    
Affiliation:
Unité Sécurité Microbiologique des Aliments, Institut des Sciences et Techniques des Aliments de Bordeaux, Ave. des Facultés, Université de Bordeaux 1, 33405 Talence, France. r.caubet@istab.u-bordeaux1.fr
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion / physiology
Biofilms* / growth & development
Culture Media
Electric Conductivity
Escherichia coli / growth & development,  pathogenicity,  physiology*
Risk Assessment
Sonication
Chemical
Reg. No./Substance:
0/Culture Media

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