Document Detail


Assessing the complex sponge microbiota: core, variable and species-specific bacterial communities in marine sponges.
MedLine Citation:
PMID:  21993395     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Marine sponges are well known for their associations with highly diverse, yet very specific and often highly similar microbiota. The aim of this study was to identify potential bacterial sub-populations in relation to sponge phylogeny and sampling sites and to define the core bacterial community. 16S ribosomal RNA gene amplicon pyrosequencing was applied to 32 sponge species from eight locations around the world's oceans, thereby generating 2567 operational taxonomic units (OTUs at the 97% sequence similarity level) in total and up to 364 different OTUs per sponge species. The taxonomic richness detected in this study comprised 25 bacterial phyla with Proteobacteria, Chloroflexi and Poribacteria being most diverse in sponges. Among these phyla were nine candidate phyla, six of them found for the first time in sponges. Similarity comparison of bacterial communities revealed no correlation with host phylogeny but a tropical sub-population in that tropical sponges have more similar bacterial communities to each other than to subtropical sponges. A minimal core bacterial community consisting of very few OTUs (97%, 95% and 90%) was found. These microbes have a global distribution and are probably acquired via environmental transmission. In contrast, a large species-specific bacterial community was detected, which is represented by OTUs present in only a single sponge species. The species-specific bacterial community is probably mainly vertically transmitted. It is proposed that different sponges contain different bacterial species, however, these bacteria are still closely related to each other explaining the observed similarity of bacterial communities in sponges in this and previous studies. This global analysis represents the most comprehensive study of bacterial symbionts in sponges to date and provides novel insights into the complex structure of these unique associations.
Authors:
Susanne Schmitt; Peter Tsai; James Bell; Jane Fromont; Micha Ilan; Niels Lindquist; Thierry Perez; Allen Rodrigo; Peter J Schupp; Jean Vacelet; Nicole Webster; Ute Hentschel; Michael W Taylor
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-10-13
Journal Detail:
Title:  The ISME journal     Volume:  6     ISSN:  1751-7370     ISO Abbreviation:  ISME J     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-14     Completed Date:  2012-09-28     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101301086     Medline TA:  ISME J     Country:  England    
Other Details:
Languages:  eng     Pagination:  564-76     Citation Subset:  IM    
Affiliation:
Centre for Microbial Innovation, School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand. s.schmitt@lrz.uni-muenchen.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteria / classification*,  genetics
Biodiversity*
DNA, Bacterial / genetics
Genes, rRNA
Geography
Metagenome*
Oceans and Seas
Phylogeny
Porifera / genetics,  microbiology*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Species Specificity
Tropical Climate
Grant Support
ID/Acronym/Agency:
S06-GM-44796/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/RNA, Ribosomal, 16S
Comments/Corrections

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