| Assessing changes in composition of intestinal microbiota in neonatal BALB/c mice through cluster analysis of molecular markers. | |
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MedLine Citation:
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PMID: 18096093 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study introduced a molecular biological approach to demonstrate changes in the composition of intestinal microbiota in neonatal mice. Female BALB/c mice were fed either a control diet or a diet supplemented with fructo-oligosaccharide (FOS) at 50 g/kg diet, and then mated to male mice. A cultivation-independent approach, denaturing gradient gel electrophoresis (DGGE) of the PCR-amplified 16S rRNA gene, was performed to characterise changes in intestinal microbial populations in pups at 0, 7, 14 and 21 d old and their dams. Comparisons of DGGE profiles were performed using the Dice similarity coefficient and the unweighted pair group method with arithmetic mean (UPGMA) cluster analysis based on numbers, positions and intensities of bands. DGGE profiles differed between dams fed control and FOS-supplemented diets. Although profiles in pups on the day of birth showed a high similarity with dams, profiles in 7-d-old pups differed from dams and showed high similarity to littermates. In 14- and 21-d-old pups, profiles again showed high similarity with dams. DGGE profiles in pups were divided into two large clusters of control and FOS-supplemented diet groups in the range of 0- to 21-d-old, suggesting modulation of intestinal microbiota in infants by manipulation of microbiota in dams. The present study shows a useful technique for demonstrating changes in intestinal microbiota and provides a mouse model for modulation of intestinal microbiota in neonatal life. |
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Authors:
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Reiko Fujiwara; Jun Watanabe; Kei Sonoyama |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The British journal of nutrition Volume: 99 ISSN: 1475-2662 ISO Abbreviation: Br. J. Nutr. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-06-04 Completed Date: 2008-06-23 Revised Date: 2009-05-20 |
Medline Journal Info:
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Nlm Unique ID: 0372547 Medline TA: Br J Nutr Country: England |
Other Details:
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Languages: eng Pagination: 1174-7 Citation Subset: IM |
Affiliation:
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Laboratory of Food Biochemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060-8589, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn / microbiology* Cluster Analysis Dietary Supplements Electrophoresis / methods Female Genes, Bacterial Genetic Markers Intestines / microbiology* Lactation* Male Maternal Nutritional Physiological Phenomena* Mice Mice, Inbred BALB C Oligosaccharides / administration & dosage RNA, Ribosomal, 16S / analysis Reverse Transcriptase Polymerase Chain Reaction / methods |
| Chemical | |
Reg. No./Substance:
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0/Genetic Markers; 0/Idolax; 0/Oligosaccharides; 0/RNA, Ribosomal, 16S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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