Document Detail


Assessing centrosomal function of infertile males using heterologous ICSI.
MedLine Citation:
PMID:  18570049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human spermatozoal centrosome acts as a microtubule organizing center and is essential for male and female pronuclear migration and apposition. In this study, we assess centrosomal function of spermatozoa from infertile patients using heterologus intracytoplasmic sperm injection (ICSI) into bovine eggs. Spermatozoa from 15 infertile patients undergoing assisted reproductive technology (ART) treatment and 3 fertile donors were tested. Microtubules and DNA were imaged by immuocytochemistry and epifluorescence microscopy. Decondensed female chromosomes and sperm nuclei, pronuclear formation and sperm aster formation were examined. The average rate of sperm aster formation using spermatozoa from infertile individuals was lower (47.0%) than that with spermatozoa from fertile individuals (66.1%). We compared the sperm aster formation rates after ART with various clinical parameters, including semen characteristics, pronuclear formation rates, embryonic cleavage rates and pregnancy outcome. Clinical semen characteristics and the rate of pronuclear formation appeared independent of sperm centrosomal function. In contrast, the centrosomal function had a substantial effect on embryonic cleavage rate and pregnancy after ART. These results suggested that centrosomal function is essential for pregnancy and embryonic development. The method described using bovine eggs is suitable to assay human centrosome function and predict pregnancy after ART.
Authors:
Tomoko Yoshimoto-Kakoi; Yukihiro Terada; Masahito Tachibana; Takashi Murakami; Nobuo Yaegashi; Kunihiro Okamura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Systems biology in reproductive medicine     Volume:  54     ISSN:  1939-6368     ISO Abbreviation:  Syst Biol Reprod Med     Publication Date:    2008 May-Jun
Date Detail:
Created Date:  2008-06-23     Completed Date:  2008-09-12     Revised Date:  2009-11-17    
Medline Journal Info:
Nlm Unique ID:  101464963     Medline TA:  Syst Biol Reprod Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  135-42     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Miyagi, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Centrosome / pathology*
Female
Fertilization in Vitro*
Humans
Immunohistochemistry
Infertility, Female / pathology*,  therapy
Infertility, Male / pathology*,  therapy
Male
Microscopy, Fluorescence
Oocytes / pathology*
Pregnancy
Pregnancy Rate
Sperm Injections, Intracytoplasmic*
Sperm-Ovum Interactions*
Spermatozoa / pathology*
Treatment Outcome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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