Document Detail


Assessing cell fusion and cytokinesis failure as mechanisms of clone 9 hepatocyte multinucleation in vitro.
MedLine Citation:
PMID:  22896007     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
In this in vitro model of hepatocyte multinucleation, separate cultures of rat Clone 9 cells are labeled with either red or green cell tracker dyes (Red Cell Tracker CMPTX or Vybrant CFDA SE Cell Tracer), plated together in mixed-color colonies, and treated with positive or negative control agents for 4 days. The fluorescent dyes become cell-impermeant after entering cells and are not transferred to adjacent cells in a population, but are inherited by daughter cells after fusion. The mixed-color cultures are then evaluated microscopically for multinucleation and analysis of the underlying mechanism (cell fusion/cytokinesis). Multinucleated cells containing only one dye have undergone cytokinesis failure, whereas dual-labeled multinucleated cells have resulted from fusion. Curr. Protoc. Toxicol. 53:14.9.1-14.9.17. © 2012 by John Wiley & Sons, Inc.
Authors:
Damir Simic; Catherine Euler; Christina Thurby; Mike Peden; Sarah Tannehill-Gregg; Todd Bunch; Thomas Sanderson; Terry Van Vleet
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]     Volume:  Chapter 14     ISSN:  1934-9262     ISO Abbreviation:  Curr Protoc Toxicol     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9816330     Medline TA:  Curr Protoc Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit14.9     Citation Subset:  IM    
Affiliation:
Drug Safety Evaluation, Bristol-Myers Squibb Co., Mount Vernon, Indiana.
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