Document Detail

Assessing cell fusion and cytokinesis failure as mechanisms of clone 9 hepatocyte multinucleation in vitro.
MedLine Citation:
PMID:  22896007     Owner:  NLM     Status:  In-Data-Review    
In this in vitro model of hepatocyte multinucleation, separate cultures of rat Clone 9 cells are labeled with either red or green cell tracker dyes (Red Cell Tracker CMPTX or Vybrant CFDA SE Cell Tracer), plated together in mixed-color colonies, and treated with positive or negative control agents for 4 days. The fluorescent dyes become cell-impermeant after entering cells and are not transferred to adjacent cells in a population, but are inherited by daughter cells after fusion. The mixed-color cultures are then evaluated microscopically for multinucleation and analysis of the underlying mechanism (cell fusion/cytokinesis). Multinucleated cells containing only one dye have undergone cytokinesis failure, whereas dual-labeled multinucleated cells have resulted from fusion. Curr. Protoc. Toxicol. 53:14.9.1-14.9.17. © 2012 by John Wiley & Sons, Inc.
Damir Simic; Catherine Euler; Christina Thurby; Mike Peden; Sarah Tannehill-Gregg; Todd Bunch; Thomas Sanderson; Terry Van Vleet
Related Documents :
18783337 - Sphingosine kinase 1 and 2 regulate the capacity of mesangial cells to resist apoptotic...
9225147 - The value of proliferating cell nuclear antigen (pcna)/cyclin in the assessment of cell...
22297447 - Embryonic and foetal islet-1 positive cells in human hearts are also positive to c-kit.
12080327 - Expression of monocyte chemoattractant protein-1 in proximal tubular epithelial cells i...
10860847 - Morphological alteration of x-ray induced partially transformed human cells by transfec...
3784577 - The application of kinematic equations for the study of cell turnover.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]     Volume:  Chapter 14     ISSN:  1934-9262     ISO Abbreviation:  Curr Protoc Toxicol     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9816330     Medline TA:  Curr Protoc Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit14.9     Citation Subset:  IM    
Drug Safety Evaluation, Bristol-Myers Squibb Co., Mount Vernon, Indiana.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Assessment of Metabolic Stability Using the Rainbow Trout (Oncorhynchus mykiss) Liver S9 Fraction.
Next Document:  Mining the salivary proteome with grating-coupled surface plasmon resonance imaging and surface plas...