Document Detail

Assessing analytical variability of measurement of vitamin A in corn-soy blend.
MedLine Citation:
PMID:  20480912     Owner:  NLM     Status:  MEDLINE    
Two multilaboratory investigations were conducted by SUSTAIN to assess variability in the measurement of vitamin A, the marker used to verify levels of vitamin premix addition to enriched/fortified food aid products, including the widely distributed corn-soy blend (CSB). CSB specifications identify AACC Approved Method 86-06 or equivalent methods for vitamin A analysis, however there is no requirement to demonstrate equivalency. CSB samples with known and blinded levels of vitamin A and a reference standard were analyzed by 16 laboratories using their respective methods. Calculated coefficients of variation across all laboratories and methods for unknown samples and reference standard were 35 and 7.1%, respectively, suggesting the largest source of variation is the vitamin extraction procedure. Laboratories generally overestimated low levels and underestimated high levels of vitamin A within the range of 6000 and 16 000 IU/lb. Only two laboratories demonstrated excellent internal precision (+/- 300 IU vitamin A/lb) and reported values within 95% confidence interval for all blinded samples. Results of this study have implications both for quality control in food aid products (due to the use of vitamin A as a marker) and for regulatory oversight of vitamin A content in commercial food products.
Wayne R Moore; Jonathan DeVries; John MacDonald; Lynne Hare; J Carson; P Chaudhari; J DeVries; J Fontana; P Golz; J King; J MacDonald; C McCullough; S Noggle; E Rasgorshek; S Schorn; L Skogerson; D Sullins; D Sullivan; M Sussman; M Weibel
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of AOAC International     Volume:  93     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-05-19     Completed Date:  2010-06-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  638-49     Citation Subset:  IM    
SUSTAIN, 2000 P St, NW, Suite 300, Washington, DC 20036, USA.
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MeSH Terms
Chemistry Techniques, Analytical
Food Analysis / methods*
Food, Fortified / analysis*
Nutritive Value
Quality Control
Reference Standards
Reproducibility of Results
Soybeans / metabolism*
United States
United States Department of Agriculture
Vitamin A / analysis,  chemistry*
Zea mays / metabolism*
Reg. No./Substance:
11103-57-4/Vitamin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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