| Assessing adhesion, biofilm formation and motility of Acidovorax citrulli using microfluidic flow chambers. | |
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MedLine Citation:
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PMID: 20807236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Acidovorax citrulli is the causal agent of bacterial fruit blotch of cucurbits. We have shown previously that type IV pili (TFP) are required for wild-type levels of virulence of A. citrulli on melon and that this pathogen can colonize and move thorough the xylem vessels of host seedlings. Here, comparative studies between wild-type and TFP mutant strains using microfluidic flow chambers demonstrated that TFP play a critical role in both the surface attachment and the biofilm formation of A. citrulli under a medium flow. Additionally, TFP null mutants were unable to perform twitching movement against the direction of medium flow. Assays using a flagellin mutant showed that, in contrast to TFP, polar flagella do not contribute to the adhesion and biofilm formation of A. citrulli under tested conditions. Also, flagellum-mediated swimming motility of wild-type strains was not observed under medium flow. These results imply that TFP may play an important role in colonization and spread in the xylem vessels under sap flow conditions, while polar flagella could be more important for spread during periods of time when xylem flow is minimal. |
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Authors:
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Ofir Bahar; Leonardo De La Fuente; Saul Burdman |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-08-27 |
Journal Detail:
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Title: FEMS microbiology letters Volume: 312 ISSN: 1574-6968 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-02 Completed Date: 2011-01-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: England |
Other Details:
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Languages: eng Pagination: 33-9 Citation Subset: IM |
Copyright Information:
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© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
Affiliation:
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Department of Plant Pathology and Microbiology and Otto Warburg Minerva Center for Agricultural Biotechnology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Adhesion* Bacterial Proteins / genetics, metabolism Biofilms* Comamonadaceae / chemistry*, genetics, physiology* Fimbriae, Bacterial / physiology Microfluidic Analytical Techniques / instrumentation, methods* Mutation |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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