Document Detail


Assessing the risks of genotoxicity in the therapeutic development of induced pluripotent stem cells.
MedLine Citation:
PMID:  23207694     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Induced pluripotent stem cells (iPSCs) have great potential for regenerative medicine as well as for basic and translational research. However, following the initial excitement over the enormous prospects of this technology, several reports uncovered serious concerns regarding its safety for clinical applications and reproducibility for laboratory applications such as disease modeling or drug screening. In particular, the genomic integrity of iPSCs is the focus of extensive research. Epigenetic remodeling, aberrant expression of reprogramming factors, clonal selection, and prolonged in vitro culture are potential pathways for acquiring genomic alterations. In this review, we will critically discuss current reprogramming technologies particularly in the context of genotoxicity, and the consequences of these alternations for the potential applications of reprogrammed cells. In addition, current strategies of genetic modification of iPSCs, as well as applicable suicide strategies to control the risk of iPSC-based therapies will be introduced.
Authors:
So Gun Hong; Cynthia E Dunbar; Thomas Winkler
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Review     Date:  2012-12-04
Journal Detail:
Title:  Molecular therapy : the journal of the American Society of Gene Therapy     Volume:  21     ISSN:  1525-0024     ISO Abbreviation:  Mol. Ther.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-01     Completed Date:  2013-08-07     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  100890581     Medline TA:  Mol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  272-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
DNA Damage*
Embryonic Stem Cells / cytology,  metabolism
Epigenomics
Gene Transfer Techniques
Genes, Transgenic, Suicide
Genetic Vectors
Genomics / methods
Humans
Induced Pluripotent Stem Cells / cytology*,  metabolism,  transplantation*
Models, Animal
Nuclear Reprogramming
Regenerative Medicine
Reproducibility of Results
Risk Factors
Translational Medical Research
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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