Document Detail


Assembly of a three-dimensional array of glycoconjugates by combinatorial biocatalysis in nonaqueous media.
MedLine Citation:
PMID:  18062672     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glycoconjugates can be artificially synthesized by combinatorial biocatalysis. An example is given in this paper describing the construction of glycoconjugates array by using glycosidase and lipase in nonaqueous media. This array was started from glucose, with three aryl alcohols as the aglycone moiety of glycosides and five acids or esters as acyl donors for combinatorial acylation of glycosides, affording a three-dimensional array containing about 30 members with diverse structures. The array would be more abundant if more aglycones and acyl donors with other structures were filled in. Indeed, diverse classes of carbohydrates besides glucose can also be employed for generating diverse glycoconjugates due to their different roles in numerous physiological responses. The composition and distribution of the demonstration glycoconjugates array was detected and evaluated by HPLC-MS with electrospray ionization. And also, the distribution of the artificial array can be adjusted by changing the molar ratio of the auxiliary materials.
Authors:
Hui-Lei Yu; Jian-He Xu; Yu-Xiao Wang; Wen-Ya Lu; Guo-Qiang Lin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-07
Journal Detail:
Title:  Journal of combinatorial chemistry     Volume:  10     ISSN:  1520-4774     ISO Abbreviation:  J Comb Chem     Publication Date:    2008 Jan-Feb
Date Detail:
Created Date:  2008-01-14     Completed Date:  2008-02-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100886263     Medline TA:  J Comb Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  79-87     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P.R. China. jianhexu@ecust.edu.cn
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MeSH Terms
Descriptor/Qualifier:
Acylation
Catalysis
Chromatography, High Pressure Liquid
Combinatorial Chemistry Techniques / methods*
Glycoconjugates / chemical synthesis*,  chemistry
Glycoside Hydrolases / chemistry*
Glycosides / chemistry*
Lipase / chemistry*
Models, Molecular
Molecular Structure
Chemical
Reg. No./Substance:
0/Glycoconjugates; 0/Glycosides; EC 3.1.1.3/Lipase; EC 3.2.1.-/Glycoside Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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