| Assembly kinetics of replicating chromatin: isolation and characterization of prenucleosomal and nucleosomal DNA. | |
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MedLine Citation:
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PMID: 6838553 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Replicating chromatin is known to be more sensitive to micrococcal nuclease than bulk chromatin. We have used this property and a fractionation procedure based on the specific release of replicating material under mild micrococcal nuclease digestion, in order to analyse both the kinetics of maturation of newly replicated DNA into nucleosomes and the structure of the replicating material. As other authors, we initially observed that repetitive unit of newly replicated chromatin was shorter than that of bulk chromatin, however this result appears to be due to sliding of nucleosomes along the chromatin fibers close to the replicating fork. Replicative chromatin was fractionated and analysed. A prenucleosomal peak was observed and preliminary characterized. |
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Authors:
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C Vaury; C Gilly; D Alix; J J Lawrence |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 110 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1983 Feb |
Date Detail:
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Created Date: 1983-05-27 Completed Date: 1983-05-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 811-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon Radioisotopes / diagnostic use Cell Line Chromatin / metabolism* Cricetinae Cricetulus DNA / genetics*, isolation & purification DNA Replication* Female Kinetics Nucleosomes / metabolism* Ovary Thymidine / metabolism Tritium / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Chromatin; 0/Nucleosomes; 10028-17-8/Tritium; 50-89-5/Thymidine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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