| Assembly and expression of a synthetic gene encoding the antigen Pfs48/45 of the human malaria parasite Plasmodium falciparum in yeast. | |
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MedLine Citation:
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PMID: 10618538 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pfs48/45 is an important transmission-blocking vaccine candidate antigen of the human malaria parasite Plasmodium falciparum. This study was aimed at synthesis of recombinant Pfs48/45 containing conformation-constrained epitopes of the native antigen in yeast. Since in the yeast Saccharomyces cerevisiae induction of gene-expression led to prematurely terminated transcripts, an entirely synthetic gene of higher GC content was assembled. Replacement of the AT rich natural gene by the synthetic gene relieved the observed premature transcription termination. Nevertheless, recombinant protein expression could not be detected. In contrast, in the yeast Pichia pastoris low levels of recombinant Pfs48/45 were produced upon induction of synthetic gene expression. The recombinant protein was shown to be disulphide-bridge constrained, but was not recognised by transmission-blocking antibodies and did not induce transmission-blocking sera in mice. |
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Authors:
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R L Milek; H G Stunnenberg; R N Konings |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Vaccine Volume: 18 ISSN: 0264-410X ISO Abbreviation: Vaccine Publication Date: 2000 Jan |
Date Detail:
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Created Date: 2000-03-07 Completed Date: 2000-03-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8406899 Medline TA: Vaccine Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1402-11 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology and Cell Biology, University of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence DNA, Protozoan / genetics Genes, Synthetic Humans Malaria / parasitology Membrane Glycoproteins / biosynthesis, genetics*, immunology, isolation & purification Molecular Sequence Data Pichia / genetics, metabolism Plasmodium falciparum / genetics* Protozoan Proteins / biosynthesis, genetics*, immunology, isolation & purification Saccharomyces cerevisiae / genetics, metabolism Transfection |
| Chemical | |
Reg. No./Substance:
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0/DNA, Protozoan; 0/Membrane Glycoproteins; 0/Protozoan Proteins; 0/pfs48-45 protein, Plasmodium falciparum |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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