Document Detail


Assembly of cytoskeletal proteins into cleavage furrows of tissue culture cells.
MedLine Citation:
PMID:  10816259     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We review results obtained after fluorescent actin and myosin II probes were microinjected into interphase and prophase PtK2 and LLC-PK tissue culture cells to follow the changing distribution of these cytoskeletal proteins in the live cells during division. The fluorescent probes first begin to assemble into the future furrow region during mid-anaphase before any sign of initial contractions. The total concentrations of F-actin and myosin in the cleavage furrow begin to decrease a few minutes after the onset of furrow contraction. The cell's shape and the position of its mitotic spindle affect the deposition of cytoskeletal proteins in the forming cleavage furrow. In cells with two spindles, contractile proteins were recruited not only to the cortex bordering the former metaphase plates but also to the cortex midway between each pair of adjacent non-daughter poles or centrosomes. The furrowing between adjacent poles seen in these cultured cells are similar to the furrows observed by Rappaport [(1961) J Exp Zool 148:81-89] when echinoderm eggs were manipulated into a torus shape so that the poles of two mitotic spindles were adjacent to one another. These observations on injected tissue culture cells suggest that vertebrate cells share common mechanisms for the establishment of the cleavage furrow with echinoderm cells.
Authors:
J M Sanger; J W Sanger
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Microscopy research and technique     Volume:  49     ISSN:  1059-910X     ISO Abbreviation:  Microsc. Res. Tech.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-07-06     Completed Date:  2000-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9203012     Medline TA:  Microsc Res Tech     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  190-201     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Wiley-Liss, Inc.
Affiliation:
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6058, USA. sangerj@mail.med.upenn.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division*
Cell Size
Cells, Cultured
Cytoskeletal Proteins / metabolism*
Mitotic Spindle Apparatus / physiology
Models, Biological
Chemical
Reg. No./Substance:
0/Cytoskeletal Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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