| Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites. | |
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MedLine Citation:
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PMID: 21868764 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Human cell lines are useful for studying cancer biology and preclinically modeling cancer therapy, but can be misidentified and cross-contamination is unfortunately common. The purpose of this study was to develop a panel of validated head and neck cell lines representing the spectrum of tissue sites and histologies that could be used for studying the molecular, genetic, and phenotypic diversity of head and neck cancer. METHODS: A panel of 122 clinically and phenotypically diverse head and neck cell lines from head and neck squamous cell carcinoma, thyroid cancer, cutaneous squamous cell carcinoma, adenoid cystic carcinoma, oral leukoplakia, immortalized primary keratinocytes, and normal epithelium was assembled from the collections of several individuals and institutions. Authenticity was verified by carrying out short tandem repeat analysis. Human papillomavirus (HPV) status and cell morphology were also determined. RESULTS: Eighty-five of the 122 cell lines had unique genetic profiles. HPV-16 DNA was detected in 2 cell lines. These 85 cell lines included cell lines from the major head and neck primary tumor sites, and close examination shows a wide range of in vitro phenotypes. CONCLUSIONS: This panel of 85 genomically validated head and neck cell lines represents a valuable resource for the head and neck cancer research community that can help advance understanding of the disease by providing a standard reference for cell lines that can be used for biological as well as preclinical studies. |
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Authors:
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Mei Zhao; Daisuke Sano; Curtis R Pickering; Samar A Jasser; Ying C Henderson; Gary L Clayman; Erich M Sturgis; Thomas J Ow; Reuben Lotan; Thomas E Carey; Peter G Sacks; Jennifer R Grandis; David Sidransky; Nils Erik Heldin; Jeffrey N Myers |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-08-25 |
Journal Detail:
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Title: Clinical cancer research : an official journal of the American Association for Cancer Research Volume: 17 ISSN: 1078-0432 ISO Abbreviation: Clin. Cancer Res. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-02 Completed Date: 2012-04-27 Revised Date: 2013-05-23 |
Medline Journal Info:
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Nlm Unique ID: 9502500 Medline TA: Clin Cancer Res Country: United States |
Other Details:
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Languages: eng Pagination: 7248-64 Citation Subset: IM |
Copyright Information:
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©2011 AACR. |
Affiliation:
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Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, 77030-4009, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alphapapillomavirus
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genetics,
isolation & purification Carcinoma, Adenoid Cystic / genetics Carcinoma, Squamous Cell* / genetics Cell Culture Techniques Cell Line, Tumor* DNA, Viral / analysis Head and Neck Neoplasms* / genetics Humans Keratinocytes / cytology Leukoplakia, Oral / genetics Microsatellite Repeats Thyroid Neoplasms / genetics |
| Grant Support | |
ID/Acronym/Agency:
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CA016672/CA/NCI NIH HHS; P30 CA016672-35/CA/NCI NIH HHS; P50 CA097007/CA/NCI NIH HHS; P50 CA097007-09/CA/NCI NIH HHS; P50CA097007/CA/NCI NIH HHS; R01 DE014613-08/DE/NIDCR NIH HHS; R01 DE014613-09/DE/NIDCR NIH HHS; R01 DE019126-04/DE/NIDCR NIH HHS; R01DE14613/DE/NIDCR NIH HHS; T32 CA060374-15/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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