Document Detail


Assembly of Bacillus subtilis FtsA: Effects of pH, ionic strength and nucleotides on FtsA assembly.
MedLine Citation:
PMID:  23036588     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In this work, the assembly of purified Bacillus subtilis FtsA was analyzed by several complimentary techniques. FtsA assembled to form filaments and bundles and the polymers disassembled upon dilution. FtsA assembled more efficiently at pH 6.0 as compared to that at pH 7.0 or 8.0 and high salt inhibited the assembly of FtsA. FtsA was found to hydrolyze ATP in vitro; however, neither ATP nor ADP influenced the assembly kinetics of FtsA. Though FtsA is a homologue of actin, cytochalasin D did not inhibit the assembly of FtsA. Interestingly, a hydrophobic molecule, 4,4'-dianilino-1,1'-binaphthyl-5,5'-disulphonic acid, inhibited the assembly of FtsA.
Authors:
Parminder Singh; Ravindra D Makde; Saikat Ghosh; Jayant Asthana; Vinay Kumar; Dulal Panda
Related Documents :
24178198 - Purification and characterization of isoforms of cinnamyl alcohol dehydrogenase from eu...
24205428 - P-i snake venom metalloproteinase is able to activate the complement system by direct c...
24973738 - Pharmacological properties and pathophysiological significance of a kunitz-type proteas...
24872208 - Bifunctional lanthanum phosphate substrates as novel adsorbents and biocatalyst support...
2674118 - Competitive inhibitors of klebsiella aerogenes urease. mechanisms of interaction with t...
18270198 - The critical role of substrate-protein hydrogen bonding in the control of regioselectiv...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-1
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  -     ISSN:  1879-0003     ISO Abbreviation:  Int. J. Biol. Macromol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Forensic medical examination of adolescent and adult victims of sexual violence.
Next Document:  Evidence for the binding affinity of daunomycin to HMGB1 protein in chromatin and in solution.