Document Detail


Assays for the quantification of melphalan and its hydrolysis products in human plasma by liquid chromatography-tandem mass spectrometry.
MedLine Citation:
PMID:  19674945     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) assays are described for the quantification of melphalan in human plasma. N-phenyldiethanolamine was tested as internal standard. The first assay consisted of a protein precipitation by cold methanol and a reversed-phase HPLC whereas the second one was based on a solid phase extraction and a hydrophilic interaction chromatography. Both provided a very satisfactory mean extraction yield with a small volume of sample. The first method was simple, rapid and used as a routine assay. The second one was developed in order to determine melphalan hydrolysis products and to avoid scarce cases when interferences from biological matrix alter the quantification of melphalan using the first method. The two assays were linear and sensitive in the range of 1-500ng/mL for the first one and in a range of 25-2000ng/mL for the second one. Concentrations out of the range fixed with the first method were also validated. The procedure was reliable with precision and accuracy below 10%. All compounds were detected after positive mode electrospray ionization in selected reaction monitoring mode. These new analytical procedures were developed for melphalan pharmacokinetic studies or therapeutic drug monitoring.
Authors:
Asmae Mirkou; Bruno Vignal; Sabine Cohen; Marc Guillaumont; Olivier Glehen; J??r??me Guitton
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Publication Detail:
Type:  Journal Article     Date:  2009-08-03
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  877     ISSN:  1873-376X     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-14     Completed Date:  2009-12-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3089-96     Citation Subset:  IM    
Affiliation:
Laboratoire de ciblage th??rapeutique en canc??rologie, Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud, F-69495 Pierre B??nite, France.
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MeSH Terms
Descriptor/Qualifier:
Aged
Chromatography, High Pressure Liquid / methods*
Drug Stability
Humans
Hydrolysis
Least-Squares Analysis
Male
Melphalan / blood*,  metabolism,  pharmacokinetics
Reproducibility of Results
Sensitivity and Specificity
Solid Phase Extraction / methods
Tandem Mass Spectrometry / methods*
Chemical
Reg. No./Substance:
148-82-3/Melphalan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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