Document Detail


Assays for inverse agonists in the visual system.
MedLine Citation:
PMID:  21050919     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Visual pigment proteins belong to the superfamily of G protein-coupled receptors and are the light-sensitive molecules in rod and cone photoreceptor cells. The protein moiety is known as opsin and the ligand in the dark is 11-cis retinal, which serves as both the photon detector and an inverse agonist. While much is known about properties of the rod pigment rhodopsin, much less is understood about cone visual pigments. Being able to identify ligands that effect opsins give an insight into structure-activity relationships. The action of some ligands indicates that there are differences between not only rod and cone opsins but also among the different classes of cone opsins. Furthermore, inverse agonists of cone opsins may have potential therapeutic uses under conditions when the native 11-cis retinal ligand is absent. A method for determining the effects of ligands on rod and cone opsin activity is described.
Authors:
Masahiro Kono
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in enzymology     Volume:  485     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-11-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  213-24     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina, USA.
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MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
R01-EY019515/EY/NEI NIH HHS

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