| Assays for the determination of HIV-1 load in semen: a review of indications, methods and performance in vitro. | |
| | |
MedLine Citation:
|
PMID: 18473781 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Techniques for the quantification of HIV-1 load in semen include culture and nucleic acid amplification techniques. The latter tend to be used in the reproductive, public health and research settings due to speed, throughput, sensitivity and capacity to eliminate and control for contamination or inhibitory substances from semen. Commercially-available assays such as nucleic acid sequence-based amplification and reverse transcriptase polymerase chain reaction are equivalent in yielding more reliable and reproducible results than in-house, non-commercial assays, and should be used for the determination of HIV-1 load in semen. Sensitivity is increased when silica extraction methods are used. |
| | |
Authors:
|
Derek J Chan; Leon McNally |
Publication Detail:
|
Type: Journal Article; Review |
Journal Detail:
|
Title: Current HIV research Volume: 6 ISSN: 1873-4251 ISO Abbreviation: Curr. HIV Res. Publication Date: 2008 May |
Date Detail:
|
Created Date: 2008-05-13 Completed Date: 2008-08-13 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101156990 Medline TA: Curr HIV Res Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 182-8 Citation Subset: IM |
Affiliation:
|
Albion Street Centre, Prince of Wales Hospital, Sydney, New South Wales, Australia. derek.chan2@sesiahs.health.nsw.gov.au |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
HIV Infections
/
transmission,
virology* HIV-1 / genetics, isolation & purification* Humans Male Polymerase Chain Reaction / methods RNA, Viral / analysis, genetics Self-Sustained Sequence Replication / methods Semen / virology* Viral Load |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: IL-1 cytokines in cardiovascular disease: diagnostic, prognostic and therapeutic implications.
Next Document: The challenge of IL-2 immunotherapy in HIV disease: "no through road" or turning point?