| Assays of d: -amino Acid oxidases. | |
| | |
MedLine Citation:
|
PMID: 21956578 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
D: -Amino acid oxidase and D: -aspartate oxidase are two well-known FAD-containing flavooxidases that catalyze the same reaction (the oxidative deamination) on different D: -amino acids. D: -aspartate oxidase is specific for acidic D: -amino acids (i.e., D: -aspartate and D: -glutamate) and D: -amino acid oxidase is active on neutral and polar D: -amino acids (a low activity is also detected on basic D: -amino acids). The assay of these flavoenzymes is of utmost importance in different fields because D: -amino acids are common constituents of bacterial cell walls, are present in foods and because free D: -serine and D: -aspartic acid were identified in brain and peripheral tissues of mammals. In this chapter, we report on the most used methods employed to assay the activity of D: -amino acid oxidase and D: -aspartate oxidase. Interestingly, their activity can be followed using different assays, namely D: -amino acid or oxygen consumption, α-keto acid or ammonia production, or using artificial dyes as final indicator of the flavin redox reaction. |
| | |
Authors:
|
Gabriella Tedeschi; Loredano Pollegioni; Armando Negri |
Related Documents
:
|
1153188 - A model for the coevolution of the genetic code and the process of protein synthesis: r... 17369878 - The genetic code--40 years on. 9687438 - De novo synthesis of amino acids by the ruminal bacteria prevotella bryantii b14, selen... 9871768 - Asymmetric synthesis of boc-n-methyl-p-benzoyl-phenylalanine. preparation of a photorea... 1153188 - A model for the coevolution of the genetic code and the process of protein synthesis: r... 341988 - Intermolecular interaction studies on native and enzyme-treated acid-soluble collagen. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 794 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
|
Created Date: 2011-09-29 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 381-95 Citation Subset: IM |
Affiliation:
|
Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria - sez. Biochimica, Università degli Studi di Milano, Milano, Italy, grabriella.tedeschi@unimi.it. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Assay of amino Acid racemases.
Next Document: Enzymes acting on d: -amino Acid containing peptides.