| Assaying tumor necrosis factor concentrations in human serum. A WHO International Collaborative study. | |
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MedLine Citation:
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PMID: 7769238 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A collaborative study involving several international research groups was conducted in order to test the validity and reproducibility of tumor necrosis factor-alpha (TNF) measurements in serum. 58 serum samples, nine of them spiked with recombinant human TNF, were aliquoted and distributed blindly to 11 different laboratories. 20 samples were obtained from cerebral malaria patients, 20 from septic shock patients, eight from patients with rheumatoid arthritis and ten from normal blood donors. The serum samples were assayed for TNF using various immunoassays (ELISA), radioimmunoassays (RIA) and bioassays. Interassay coefficient of variance was analysed. Substantial differences were observed on terms of sensitivity and results obtained with the different commercial and in-house assays. The level of sensitivity was highest with ELISAs and bioassays. RIAs yielded the highest concentrations of TNF in the same samples as compared to those obtained by ELISAs and bioassays. These data emphasize the necessity of establishing international standards for cytokine assays in order to render the interpretation of biological and medical data more reliable. |
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Authors:
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S de Kossodo; V Houba; G E Grau |
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Publication Detail:
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Type: Journal Article; Multicenter Study |
Journal Detail:
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Title: Journal of immunological methods Volume: 182 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 1995 May |
Date Detail:
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Created Date: 1995-06-30 Completed Date: 1995-06-30 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 107-14 Citation Subset: IM |
Affiliation:
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Department of Dermatology, UT Southwestern Medical Center, Dallas, TX, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arthritis, Rheumatoid
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immunology Biological Assay / methods Enzyme-Linked Immunosorbent Assay / methods Humans Malaria, Cerebral / immunology Radioimmunoassay / methods Shock, Septic / immunology Tumor Necrosis Factor-alpha / analysis* World Health Organization |
| Chemical | |
Reg. No./Substance:
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0/Tumor Necrosis Factor-alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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