Document Detail


Assaying proteases in cellular environments.
MedLine Citation:
PMID:  18429231     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To better study protease activation, regulation, and inhibition physiologically or pharmacologically, it is often advantageous to use cell-based systems. The introduction of the cellular environment poses additional challenges for the assay of the protease of interest. In this unit, various methods for monitoring protease activity in cell-based systems is discussed. The scope of the unit is restricted to mammalian cell system. It is also stressed that specific considerations or requirements for a particular protease or peptidase are often needed in order to optimize the assay.
Authors:
Kevin K W Wang
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in protein science / editorial board, John E. Coligan ... [et al.]     Volume:  Chapter 21     ISSN:  1934-3663     ISO Abbreviation:  -     Publication Date:  2002 May 
Date Detail:
Created Date:  2008-04-22     Completed Date:  2008-05-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101287868     Medline TA:  Curr Protoc Protein Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 21.12     Citation Subset:  IM    
Affiliation:
Pfizer Global Research and Development, Ann Arbor, Michigan, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells / enzymology
Enzyme Activation
Humans
Methods
Peptide Hydrolases / analysis*,  metabolism*
Protease Inhibitors
Chemical
Reg. No./Substance:
0/Protease Inhibitors; EC 3.4.-/Peptide Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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