Document Detail


Assaying microRNA loss-of-function phenotypes in mammalian cells: emerging tools and their potential therapeutic utility.
MedLine Citation:
PMID:  19901530     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MicroRNAs are small, non-coding RNAs that are increasingly appreciated to play critical roles in the modulation of gene expression. In mammalian cells, our knowledge regarding the full impact of microRNAs on cellular behavior remains fragmentary. This has been due, in significant part, to the limited availability of experimental tools for studying microRNA loss-of-function phenotypes. Recently, several strategies for achieving this goal have been developed. Here, we discuss these methodologies for inhibiting specific microRNAs in mammalian cells both in vitro and in vivo, compare and contrast the strengths and weaknesses of these approaches, and speculate regarding the future impact of these antagonists on the treatment of human diseases such as cancer. These emerging techniques enable the attenuation of microRNA function in a manner that is quite sequence-specific, relatively long-lasting and increasingly cost-effective. As such, some of these advances hold great promise in terms of their eventual utility as therapeutic agents.
Authors:
Scott Valastyan; Robert A Weinberg
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review     Date:  2009-11-14
Journal Detail:
Title:  RNA biology     Volume:  6     ISSN:  1555-8584     ISO Abbreviation:  RNA Biol     Publication Date:    2009 Nov-Dec
Date Detail:
Created Date:  2009-12-17     Completed Date:  2010-04-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101235328     Medline TA:  RNA Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  541-5     Citation Subset:  IM    
Affiliation:
Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells
Drug Therapy
Humans
MicroRNAs / antagonists & inhibitors*
Neoplasms / drug therapy
Grant Support
ID/Acronym/Agency:
R01 CA078461/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/MicroRNAs

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