| Assaying CO2 release for determination of formate dehydrogenase activity in entrapment matrices and aqueous-organic two-phase systems. | |
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MedLine Citation:
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PMID: 16586512 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Formate dehydrogenase is an important enzyme for NADH-regeneration in enzyme-catalysed reductions. Methods to determine the activity of this biocatalyst during reaction in aqueous-organic two-phase systems or after immobilisation were therefore investigated. Determination of gaseous CO2 in the headspace of reaction vessels either by gas chromatography or by pressure sensors was found to be a suitable way for deduction of FDH-activity in either of these reaction systems. In the presence of organic solvents, gas chromatography yielded more precise data than pressure sensors, while pressure measurements offer the opportunity to assay continuously the activity of entrapped FDH throughout the whole course of reaction. |
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Authors:
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Marion B Ansorge-Schumacher; Sonja Steinsiek; Werner Eberhard; Nikolaos Keramidas; Klaus Erkens; Winfried Hartmeier; Jochen Büchs |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 95 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-08-28 Completed Date: 2006-10-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 199-203 Citation Subset: IM |
Copyright Information:
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(c) 2006 Wiley Periodicals, Inc. |
Affiliation:
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Department of Biotechnology, RWTH Aachen University, D-52056 Aachen, Germany. m.ansorge@biotec.rwth-aachen.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms* Candida / enzymology* Carbon Dioxide / isolation & purification* Chromatography, Gas / methods* Enzyme Activation Enzymes, Immobilized / analysis, chemistry Formate Dehydrogenases / analysis*, chemistry* Organic Chemicals / chemistry Phase Transition Water / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Enzymes, Immobilized; 0/Organic Chemicals; 124-38-9/Carbon Dioxide; 7732-18-5/Water; EC 1.2.1.2/Formate Dehydrogenases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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