Document Detail


Assaying CO2 release for determination of formate dehydrogenase activity in entrapment matrices and aqueous-organic two-phase systems.
MedLine Citation:
PMID:  16586512     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Formate dehydrogenase is an important enzyme for NADH-regeneration in enzyme-catalysed reductions. Methods to determine the activity of this biocatalyst during reaction in aqueous-organic two-phase systems or after immobilisation were therefore investigated. Determination of gaseous CO2 in the headspace of reaction vessels either by gas chromatography or by pressure sensors was found to be a suitable way for deduction of FDH-activity in either of these reaction systems. In the presence of organic solvents, gas chromatography yielded more precise data than pressure sensors, while pressure measurements offer the opportunity to assay continuously the activity of entrapped FDH throughout the whole course of reaction.
Authors:
Marion B Ansorge-Schumacher; Sonja Steinsiek; Werner Eberhard; Nikolaos Keramidas; Klaus Erkens; Winfried Hartmeier; Jochen Büchs
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  95     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-28     Completed Date:  2006-10-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  199-203     Citation Subset:  IM    
Copyright Information:
(c) 2006 Wiley Periodicals, Inc.
Affiliation:
Department of Biotechnology, RWTH Aachen University, D-52056 Aachen, Germany. m.ansorge@biotec.rwth-aachen.de
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MeSH Terms
Descriptor/Qualifier:
Algorithms*
Candida / enzymology*
Carbon Dioxide / isolation & purification*
Chromatography, Gas / methods*
Enzyme Activation
Enzymes, Immobilized / analysis,  chemistry
Formate Dehydrogenases / analysis*,  chemistry*
Organic Chemicals / chemistry
Phase Transition
Water / chemistry
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Organic Chemicals; 124-38-9/Carbon Dioxide; 7732-18-5/Water; EC 1.2.1.2/Formate Dehydrogenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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