Document Detail


Assay for opsonin activity based on chemiluminescence of a Cypiridina luciferin analog.
MedLine Citation:
PMID:  1610955     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Opsonin activity (OA) was investigated in serum samples from cord blood of 11 full-term newborn infants and 12 healthy adult volunteers by the method of luminol-dependent chemiluminescence (L-CL) and Cypiridina luciferin analog-dependent chemiluminescence (CLA-CL) together with the measurement of complement components (C3, C4, C3A and properdin). CL and complement components of cord samples were significantly lower than that of healthy adult blood. CLA-CL showed a significant correlationship to C3 and C3A. L-CL also displayed a significant correlationship to C3A but the relationship to C3 was not significant. From these results, when OA is measured by CL, CLA-CL is preferred to L-CL as a technique.
Authors:
A Nishida; H Kimura; M Nakano
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biology of the neonate     Volume:  61     ISSN:  0006-3126     ISO Abbreviation:  Biol. Neonate     Publication Date:  1992  
Date Detail:
Created Date:  1992-07-24     Completed Date:  1992-07-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0247551     Medline TA:  Biol Neonate     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  253-8     Citation Subset:  IM    
Affiliation:
Neonatal Unit, Children's Hospital Hachiouji, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Complement C3 / metabolism
Complement C3a / metabolism
Complement C4 / metabolism
Fetal Blood / metabolism
Humans
Infant, Newborn
Luminescent Measurements*
Luminol / pharmacology
Opsonin Proteins / blood*
Properdin / metabolism
Pyrazines / pharmacology*
Chemical
Reg. No./Substance:
0/Complement C3; 0/Complement C4; 0/Opsonin Proteins; 0/Pyrazines; 102146-00-9/2-methyl-6-phenyl-3,7-dihydroimidazo(1,2-a)pyrazin-3-one; 11016-39-0/Properdin; 521-31-3/Luminol; 80295-42-7/Complement C3a

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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