| Assay of gamma-glutamyltransferase with amino acid dehydrogenases from Bacillus stearothermophilus as auxiliary enzymes. | |
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MedLine Citation:
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PMID: 1350523 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A spectrophotometric assay for serum gamma-glutamyltransferase (gamma-GT, EC 2.3.2.2) based on the increasing absorbance at 340 nm due to NADH formation is described. Using gamma-glutamyl dipeptides as the donor substrate, amino acids formed by the gamma-glutamyl transfer from the donor substrate to the acceptor substrate glycylglycine are stoichiometrically converted by the corresponding amino acid dehydrogenases from Bacillus stearothermophilus to produce their keto acids and NADH. gamma-Glutamylalanine was found to be the most specific and sensitive donor substrate. The method can be mechanized, is free of interference and correlates well with conventional methods. |
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Authors:
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H Kondo; M Hashimoto; K Nagata; K Tomita; H Tsubota |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 207 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1992 Apr |
Date Detail:
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Created Date: 1992-07-02 Completed Date: 1992-07-02 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 1-9 Citation Subset: IM |
Affiliation:
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Research and Development Center, UNITIKA Ltd., Kyoto, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Oxidoreductases
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metabolism* Geobacillus stearothermophilus / enzymology* Indicators and Reagents / standards Spectrophotometry, Ultraviolet gamma-Glutamyltransferase / blood*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; EC 1.4.-/Amino Acid Oxidoreductases; EC 2.3.2.2/gamma-Glutamyltransferase |
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