Document Detail


Assay and functional properties of SopE in the recruitment of Rab5 on Salmonella-containing phagosomes.
MedLine Citation:
PMID:  16473596     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the mechanism by which Salmonella inhibits transport to lysosomes to survive in macrophages. We have purified the Salmonella-containing phagosomes from macrophages and determined the presence of different endocytic Rab proteins on the phagosomes. Our results have shown that live Salmonella-containing phagosomes recruit more Rab5 than dead Salmonella-containing phagosomes. Recruitment of Rab5 on live Salmonella-containing phagosomes depends on the presence of viable bacteria in the phagosomes. Subsequently, we identified an effector molecule of Salmonella, SopE, which specifically binds Rab5. Moreover, SopE is found to be a specific nucleotide exchange factor for Rab5 and thereby retains Rab5 in an active conformation. Activated Rab5 on Salmonella-containing phagosomes promotes fusion with early endosomes and thus avoids transport to the lysosomes.
Authors:
Seetharaman Parashuraman; Amitabha Mukhopadhyay
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in enzymology     Volume:  403     ISSN:  0076-6879     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2005  
Date Detail:
Created Date:  2006-02-13     Completed Date:  2006-04-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  295-309     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / metabolism,  physiology*
Cell Line
Mice
Phagosomes / microbiology*
Protein Binding
Salmonella / isolation & purification*
rab5 GTP-Binding Proteins / metabolism,  physiology*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/SopE protein, Salmonella; EC 3.6.5.2/rab5 GTP-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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