| Assay of amino Acid racemases. | |
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MedLine Citation:
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PMID: 21956577 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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D: -Amino acids play important physiological roles in the mammalian body. Recent investigations revealed that, in mammals, D: -amino acids are synthesized from their corresponding L: -enantiomers via amino acid racemase. This article describes a method used to measure amino acid racemase activity by high-performance liquid chromatography (HPLC). The assay involves fluorogenic chiral derivatization of amino acids with a newly developed reagent, and enantioseparation of D: - and L: -amino acid derivatives by HPLC. The method is accurate and reliable, and can be automated using a programmable autosampling injector. |
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Authors:
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Masumi Katane; Masae Sekine; Hiroshi Homma |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 794 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2011-09-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 367-79 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical Life Sciences, Kitasato University, Minatoku, Tokyo, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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