Document Detail


Assay for D-penicillamine-protein conjugate in human plasma utilising chemical reduction followed by high-performance liquid chromatography with gold/mercury electrochemical detection.
MedLine Citation:
PMID:  3235506     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
D-Penicillamine (D-pen), a thiol with antirheumatic activity, forms a mixed disulphide with albumin in vivo. This conjugate is important in the pharmacokinetics and possibly the mode of action of D-pen. An assay was devised for D-pen-albumin disulphide, based on separation from plasma by acid precipitation followed by quantitative reduction with sodium borohydride in an anoxic environment. The liberated D-pen was then assayed by high-performance liquid chromatography with gold/mercury electrochemical detection. The assay was sensitive to 1.2-microM D-pen-albumin disulphide (signal-to-noise ratio greater than 2), absolute recovery was 92.7% and intra-assay coefficient of variation was 4.6% in human plasma. This technique also may be useful for quantitating protein conjugates of other thiols.
Authors:
D A Joyce; D N Wade
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  430     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1989-05-10     Completed Date:  1989-05-10     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  319-27     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Arthritis, Rheumatoid / blood
Blood Proteins / metabolism*
Borohydrides
Chromatography, High Pressure Liquid
Disulfides / analysis
Electrochemistry
Gold / analysis
Humans
Mercury / analysis
Oxidation-Reduction
Penicillamine / blood*
Protein Binding
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/Borohydrides; 0/Disulfides; 7440-57-5/Gold; FXS1BY2PGL/Mercury; GNN1DV99GX/Penicillamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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