Document Detail

Assay for D-penicillamine-protein conjugate in human plasma utilising chemical reduction followed by high-performance liquid chromatography with gold/mercury electrochemical detection.
MedLine Citation:
PMID:  3235506     Owner:  NLM     Status:  MEDLINE    
D-Penicillamine (D-pen), a thiol with antirheumatic activity, forms a mixed disulphide with albumin in vivo. This conjugate is important in the pharmacokinetics and possibly the mode of action of D-pen. An assay was devised for D-pen-albumin disulphide, based on separation from plasma by acid precipitation followed by quantitative reduction with sodium borohydride in an anoxic environment. The liberated D-pen was then assayed by high-performance liquid chromatography with gold/mercury electrochemical detection. The assay was sensitive to 1.2-microM D-pen-albumin disulphide (signal-to-noise ratio greater than 2), absolute recovery was 92.7% and intra-assay coefficient of variation was 4.6% in human plasma. This technique also may be useful for quantitating protein conjugates of other thiols.
D A Joyce; D N Wade
Related Documents :
25435236 - Direct-injection chemiluminescence detector. properties and potential applications in f...
24079166 - Improved extraction and complete mass spectral characterization of steroidal alkaloids ...
24771656 - Chiral ligand-exchange resolution of underivatized amino acids on a dynamically modifie...
23625436 - Biological activities and chemical composition of the stems and roots of helichrysum ol...
17363156 - Different methods in tio2 photodegradation mechanism studies: gaseous and tio2-adsorbed...
15890546 - Characteristics of peripheral microembolization during iliac stenting: doppler ultrasou...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  430     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1989-05-10     Completed Date:  1989-05-10     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  319-27     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Arthritis, Rheumatoid / blood
Blood Proteins / metabolism*
Chromatography, High Pressure Liquid
Disulfides / analysis
Gold / analysis
Mercury / analysis
Penicillamine / blood*
Protein Binding
Reg. No./Substance:
0/Blood Proteins; 0/Borohydrides; 0/Disulfides; 7440-57-5/Gold; FXS1BY2PGL/Mercury; GNN1DV99GX/Penicillamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Assay for enkephalin-degrading peptidases in rat brain tissues by high-performance liquid chromatogr...
Next Document:  Purification of ascitic fluid-derived murine monoclonal antibodies by anion-exchange and size-exclus...