| Assay for D-penicillamine-protein conjugate in human plasma utilising chemical reduction followed by high-performance liquid chromatography with gold/mercury electrochemical detection. | |
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MedLine Citation:
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PMID: 3235506 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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D-Penicillamine (D-pen), a thiol with antirheumatic activity, forms a mixed disulphide with albumin in vivo. This conjugate is important in the pharmacokinetics and possibly the mode of action of D-pen. An assay was devised for D-pen-albumin disulphide, based on separation from plasma by acid precipitation followed by quantitative reduction with sodium borohydride in an anoxic environment. The liberated D-pen was then assayed by high-performance liquid chromatography with gold/mercury electrochemical detection. The assay was sensitive to 1.2-microM D-pen-albumin disulphide (signal-to-noise ratio greater than 2), absolute recovery was 92.7% and intra-assay coefficient of variation was 4.6% in human plasma. This technique also may be useful for quantitating protein conjugates of other thiols. |
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Authors:
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D A Joyce; D N Wade |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography Volume: 430 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1988 Sep |
Date Detail:
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Created Date: 1989-05-10 Completed Date: 1989-05-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 319-27 Citation Subset: IM |
Affiliation:
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University of New South Wales, School of Physiology and Pharmacology, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arthritis, Rheumatoid
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blood Blood Proteins / metabolism* Borohydrides Chromatography, High Pressure Liquid Disulfides / analysis Electrochemistry Gold / analysis Humans Mercury / analysis Oxidation-Reduction Penicillamine / blood* Protein Binding |
| Chemical | |
Reg. No./Substance:
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0/Blood Proteins; 0/Borohydrides; 0/Disulfides; 52-67-5/Penicillamine; 7439-97-6/Mercury; 7440-57-5/Gold |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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