Document Detail

Aspiration of equine oocytes from immature follicles after treatment with equine pituitary extract (EPE) alone or in combination with hCG.
MedLine Citation:
PMID:  18930362     Owner:  NLM     Status:  MEDLINE    
This study examined the effect of treating mares with equine pituitary extract (EPE) alone or in combination with hCG on the recovery rate of immature follicles by transvaginal follicular aspiration (ovum pick-up; OPU). Ten normally cycling crossbred mares aged 3-15 years and weighing 350-400 kg were subjected to each of three treatments in a random sequence with each exposure to a new treatment separated by a rest cycle during which a spontaneous ovulation occurred. The treatments were (1) superovulated with 25mg EPE and treated with 2500 IU hCG, (2) superovulation with 25mg EPE, and (3) control (no exogenous treatment). Treatments 7 days after spontaneous ovulation; and all the follicles >10mm were aspirated 24h after the largest follicle achieved a diameter of 27-30 mm for control group, and most follicles reached 22-27 mm for the EPE alone treatment. To the group EPE+hCG, when the follicles reached 22-27 mm, hCG was administered, 24h before OPU. Superovulation increased the number of follicles available for aspiration. The total number of follicles available for aspiration was 61 in the EPE/hCG group, 63 in the EPE group and 42 in the control. The proportion of follicles aspirated varied from 63.5% to 73.8%. Oocyte recovery rate ranged from 15.0% to 16.7% and the proportion of mares that yielded at least one oocyte was 70% (7/10) in the EPE/hCG, 60% (6/10) in the EPE alone and 50% (5/10) in control group. The EPE/hCG treatment had a higher proportion of follicles with expanded granulose cells (64.4%) than the control (3.3%; p<0.05) and the EPE treatment (25.0%). The intervals from spontaneous ovulation to aspiration were similar for all treatments (11-12 days). However, superovulatory treatment significantly increased the aspiration to ovulation interval from 15+/-4 days for control to 27+/-15 days for EPE (p<0.05) and to 23+/-13 days for EPE/hCG treatment with commensurate increases in the time between spontaneous ovulations.
I D P Blanco; L G Devito; H N Ferreira; G H M Araujo; C B Fernandes; M A Alvarenga; F C Landim-Alvarenga
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial     Date:  2008-09-12
Journal Detail:
Title:  Animal reproduction science     Volume:  114     ISSN:  1873-2232     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-06-02     Completed Date:  2009-09-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  203-9     Citation Subset:  IM    
Department Animal Reproduction and Veterinary Radiology, FMVZ - UNESP - Botucatu, SP-18618.000, Brazil.
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MeSH Terms
Chorionic Gonadotropin / administration & dosage,  pharmacology*
Cross-Over Studies
Drug Therapy, Combination
Oocytes / physiology*
Ovarian Follicle / drug effects*
Pituitary Gland / physiology*
Tissue Extracts / administration & dosage,  pharmacology*
Tissue and Organ Harvesting / methods,  veterinary
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Tissue Extracts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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