Document Detail


Aspergillus volatiles regulate aflatoxin synthesis and asexual sporulation in Aspergillus parasiticus.
MedLine Citation:
PMID:  17890344     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aspergillus parasiticus is one primary source of aflatoxin contamination in economically important crops. To prevent the potential health and economic impacts of aflatoxin contamination, our goal is to develop practical strategies to reduce aflatoxin synthesis on susceptible crops. One focus is to identify biological and environmental factors that regulate aflatoxin synthesis and to manipulate these factors to control aflatoxin biosynthesis in the field or during crop storage. In the current study, we analyzed the effects of aspergillus volatiles on growth, development, aflatoxin biosynthesis, and promoter activity in the filamentous fungus A. parasiticus. When colonies of Aspergillus nidulans and A. parasiticus were incubated in the same growth chamber, we observed a significant reduction in aflatoxin synthesis and asexual sporulation by A. parasiticus. Analysis of the headspace gases demonstrated that A. nidulans produced much larger quantities of 2-buten-1-ol (CA) and 2-ethyl-1-hexanol (EH) than A. parasiticus. In its pure form, EH inhibited growth and increased aflatoxin accumulation in A. parasiticus at all doses tested; EH also stimulated aflatoxin transcript accumulation. In contrast, CA exerted dose-dependent up-regulatory or down-regulatory effects on aflatoxin accumulation, conidiation, and aflatoxin transcript accumulation. Experiments with reporter strains carrying nor-1 promoter deletions and mutations suggested that the differential effects of CA were mediated through separate regulatory regions in the nor-1 promoter. The potential efficacy of CA as a tool for analysis of transcriptional regulation of aflatoxin biosynthesis is discussed. We also identify a novel, rapid, and reliable method to assess norsolorinic acid accumulation in solid culture using a Chroma Meter CR-300 apparatus.
Authors:
Ludmila V Roze; Randolph M Beaudry; Anna E Arthur; Ana M Calvo; John E Linz
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Publication Detail:
Type:  Journal Article     Date:  2007-09-21
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  73     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-11-12     Completed Date:  2008-02-11     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7268-76     Citation Subset:  IM    
Affiliation:
Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USA.
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MeSH Terms
Descriptor/Qualifier:
Aflatoxins / biosynthesis*
Aspergillus / genetics,  metabolism*,  physiology
Carbon Dioxide / chemistry,  metabolism,  pharmacology
Chromatography, Thin Layer
Cyclic AMP / metabolism
Ethylenes / chemistry,  metabolism,  pharmacology
Gene Expression Regulation, Fungal / drug effects
Hexanols / chemistry,  metabolism,  pharmacology
Mycelium / drug effects,  genetics,  metabolism
Oxygen / chemistry,  metabolism,  pharmacology
RNA, Fungal / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Spores, Fungal / drug effects,  genetics,  growth & development*
Volatilization
Chemical
Reg. No./Substance:
0/Aflatoxins; 0/Ethylenes; 0/Hexanols; 0/RNA, Fungal; 124-38-9/Carbon Dioxide; 60-92-4/Cyclic AMP; 7782-44-7/Oxygen; 91GW059KN7/ethylene; XZV7TAA77P/2-ethylhexanol
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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