Document Detail


Aspergillus fumigatus challenge increases cytokine levels in nasal lavage fluid.
MedLine Citation:
PMID:  16966303     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several studies have shown an association between exposure in moisture-damaged buildings and adverse health effects. There are several indicator microbes of moisture damage, but Aspergillus fumigatus is one of the best-documented molds provoking health problems in different occupational conditions. We assessed whether inhalation of a commercial A. fumigatus solution would affect cytokine levels (tumor necrosis factor [TNF]-alpha, interleukin [IL]-1beta, IL-4, IL-6, interferon [IFN]-gamma) in nasal lavage fluid (NAL) compared with that evoked by placebo challenge. Twenty-seven subjects were studied: 13 had occupational exposure in a moisture-damaged building, 4 were atopic, and 10 were considered as controls. In all the subjects, the IL-1beta levels were increased significantly both at 6 (p = 0.013) and 24 h (p = .005) after the A. fumigatus challenge compared to placebo. In subjects with previous occupational exposure in a moisture-damaged building, IL-4 concentrations were increased significantly both at 6 (p =.046) and 24 h (p =.008) after the A. fumigatus challenge compared with placebo. Furthermore, in the control group, TNF-alpha levels were significantly increased at 6 h after the A. fumigatus challenge compared to placebo (p = .028). Thus, these data show a link between markers of inflammation in NAL and experimental A. fumigatus challenge.
Authors:
H Stark; M Roponen; M Purokivi; J Randell; H Tukiainen; M-R Hirvonen
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Publication Detail:
Type:  Comparative Study; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Inhalation toxicology     Volume:  18     ISSN:  1091-7691     ISO Abbreviation:  Inhal Toxicol     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-09-12     Completed Date:  2006-09-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8910739     Medline TA:  Inhal Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1033-9     Citation Subset:  IM    
Affiliation:
Department of Respiratory Medicine, Kuopio University Hospital, Kuopio, Finland. harri.stark@kuh.fi
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MeSH Terms
Descriptor/Qualifier:
Adult
Air Microbiology
Aspergillus fumigatus / pathogenicity*
Female
Humans
Humidity
Inflammation Mediators / analysis*,  metabolism
Inhalation Exposure*
Interferon-gamma / analysis*,  biosynthesis
Interleukin-1 / analysis*,  biosynthesis
Interleukin-4 / analysis*,  biosynthesis
Middle Aged
Nasal Lavage Fluid / chemistry*,  cytology,  microbiology
Occupational Exposure
Time Factors
Tumor Necrosis Factor-alpha / analysis*,  biosynthesis
Chemical
Reg. No./Substance:
0/IL4 protein, human; 0/Inflammation Mediators; 0/Interleukin-1; 0/Tumor Necrosis Factor-alpha; 207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma

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