Document Detail


Aspartic proteinases in gastric mucosa of the rat: absence of pepsinogen I, genetic polymorphism of pepsinogen II, and presence of slow-moving proteinase.
MedLine Citation:
PMID:  3292334     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have examined relationships among the aspartic proteinases in rat and human gastric mucosa by electrophoretic analysis in polyacrylamide gel and by immunoblotting and immunohistochemical staining using rabbit antisera to human pepsinogen I (PG I), pepsinogen II (PG II), and slow-moving proteinase. By electrophoretic analysis, the major proteolytic bands in mucosal extracts from each of three strains of rats had rates of anodal migration that were similar to the fastest migrating isozymogens of human PG I. However, immunoblots revealed that these bands and several minor proteolytic bands with slower rates of anodal migration reacted with antiserum to PG II. Two proteolytic bands in rat gastric mucosa that migrated concurrently with human slow-moving proteinase reacted with antihuman slow-moving proteinase reacted with antihuman slow-moving proteinase. None of the proteolytic bands in rat gastric mucosa reacted with anti-PG I. By immunohistochemical staining, anti-PG I failed to stain any cells in rat fundic gland or antral mucosa. By contrast, anti-PG II stained mucus neck and chief cells in fundic gland mucosa and pyloric gland cells in antral mucosa, and anti-slow-moving proteinase stained surface and foveolar epithelial cells throughout the stomach. The results indicate that the gastric mucosa of the rat does not contain PG I.
Authors:
K H Lai; J B Wyckoff; I M Samloff
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Gastroenterology     Volume:  95     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1988-08-12     Completed Date:  1988-08-12     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  295-301     Citation Subset:  AIM; IM    
Affiliation:
Research Service, Veterans Administration Medical Center, Sepulveda, California.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aspartic Acid Endopeptidases
Electrophoresis, Polyacrylamide Gel
Endopeptidases / isolation & purification*
Gastric Mucosa / enzymology*
Humans
Immunohistochemistry
Pepsinogens / genetics,  isolation & purification*
Polymorphism, Genetic
Rats
Grant Support
ID/Acronym/Agency:
AM 172337/AM/NIADDK NIH HHS; AM 32015/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Pepsinogens; EC 3.4.-/Endopeptidases; EC 3.4.23.-/Aspartic Acid Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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